Anti-Ago1 antibody (ab223644)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Ago1 antibody
See all Ago1 primary antibodies -
Description
Goat polyclonal to Ago1 -
Host species
Goat -
Specificity
ab223644 is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Ago1 aa 382-395 (internal sequence) (Cysteine residue). (NP_036331.1).
Sequence:C-KNASYNLDPYIQEF
Database link: Q9UL18 -
Positive control
- WB: HEK-293 lysate overexpressing human Ago1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab223644 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab223644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 97 kDa. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 26523 Human
- Entrez Gene: 236511 Mouse
- Omim: 606228 Human
- SwissProt: Q9UL18 Human
- SwissProt: Q8CJG1 Mouse
- Unigene: 22867 Human
- Unigene: 30800 Mouse
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Alternative names
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
Images
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All lanes : Anti-Ago1 antibody (ab223644) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human Ago1
Lane 2 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab223644 has not yet been referenced specifically in any publications.