Overview

  • Product name

  • Description

    Goat polyclonal to Ago1
  • Host species

    Goat
  • Specificity

    ab223644 is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Ago1 aa 382-395 (internal sequence) (Cysteine residue). (NP_036331.1).
    Sequence:

    C-KNASYNLDPYIQEF


    Database link: Q9UL18

  • Positive control

    • WB: HEK-293 lysate overexpressing human Ago1.

Properties

Applications

Our Abpromise guarantee covers the use of ab223644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 97 kDa.

Target

  • Function

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similarities

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization

    Cytoplasm > P-body.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGO1 antibody
    • AGO1_HUMAN antibody
    • Argonaute 1 antibody
    • Argonaute 1 RISC catalytic component antibody
    • Argonaute1 antibody
    • eIF 2C 1 antibody
    • eIF-2C 1 antibody
    • eIF2C 1 antibody
    • EIF2C antibody
    • Eif2c1 antibody
    • Eukaryotic translation initiation factor 2C 1 antibody
    • GERP95 antibody
    • Golgi Endoplasmic Reticulum protein 95 kDa antibody
    • hAgo1 antibody
    • Protein argonaute 1 antibody
    • Protein argonaute-1 antibody
    • Putative RNA binding protein Q99 antibody
    • Putative RNA-binding protein Q99 antibody
    • Q99 antibody
    see all

Images

  • All lanes : Anti-Ago1 antibody (ab223644) at 1 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human Ago1
    Lane 2 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate

    Predicted band size: 97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

References

ab223644 has not yet been referenced specifically in any publications.

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