Overview

  • Product name
  • Description
    Rabbit polyclonal to Ago1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) Ago1.

    (Peptide available as ab23538.)

  • Positive control
    • This antibody gave a positive signal in Drosophila embryo nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab5070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 18463631
IHC-P Use at an assay dependent concentration. PubMed: 18528333
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

Target

  • Function
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similarities
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute 1 RISC catalytic component antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    Images

    • Anti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg

      Secondary
      Sheep anti-rabbit HRP conjugated IgG Polyclonal

      Performed under reducing conditions.

      Predicted band size: 110 kDa



      Blot was blocked with 5% Milk for 1 hour at room temperature.

      See Abreview

    • Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 110 kDa
      Observed band size: 110 kDa
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    References

    This product has been referenced in:
    • Mondal T  et al. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS One 13:e0190548 (2018). Read more (PubMed: 29385168) »
    • Zhang X  et al. Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle ofAedes aegypti. Proc Natl Acad Sci U S A 114:E1895-E1903 (2017). Read more (PubMed: 28223504) »

    See all 34 Publications for this product

    Customer reviews and Q&As

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    300000 cells
    Specification
    A549 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (embryonic cells)
    Total protein in input
    1e+006 cells
    Specification
    embryonic cells
    Immuno-precipitation step
    Protein A
    Username

    Abcam user community

    Verified customer

    Submitted May 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (many whole cells)
    Loading amount
    15 µg
    Specification
    many whole cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryo)
    Total protein in input
    1 µg
    Specification
    Embryo
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2010

    Application
    Western blot
    Sample
    Schmidtea mediterranea Tissue lysate - whole (many adult somatic stem cells)
    Loading amount
    21 µg
    Specification
    many adult somatic stem cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Dr. An Zeng

    Verified customer

    Submitted Mar 12 2010

    Thank you for your enquiry. No, to our knowledge this antiserum has not yet been applied using immunoprecipitation. I would be very interested to know how it performs and therefore I am prepared to provide you with a discount code should you wish to...

    Read More

    Thank you for your enquiry. Lot# 64529 is no longer available, but the current stock that we do have is from the same rabbit. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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