• Product name
  • Description
    Rabbit polyclonal to Ago1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI) of Human Ago1 (NP_036331).

  • Positive control
    • THP1 cell lysate



Our Abpromise guarantee covers the use of ab98056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.


  • Function
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similarities
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGO1 antibody
    • AGO1_HUMAN antibody
    • Argonaute 1 antibody
    • Argonaute 1 RISC catalytic component antibody
    • Argonaute1 antibody
    • eIF 2C 1 antibody
    • eIF-2C 1 antibody
    • eIF2C 1 antibody
    • EIF2C antibody
    • Eif2c1 antibody
    • Eukaryotic translation initiation factor 2C 1 antibody
    • GERP95 antibody
    • Golgi Endoplasmic Reticulum protein 95 kDa antibody
    • hAgo1 antibody
    • Protein argonaute 1 antibody
    • Protein argonaute-1 antibody
    • Putative RNA binding protein Q99 antibody
    • Putative RNA-binding protein Q99 antibody
    • Q99 antibody
    see all


  • Anti-Ago1 antibody (ab98056) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 97 kDa

    Gel concentration: 6-18%
  • ab98056 staining Ago1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and blocked with 10% serum for 12 hours at 4°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS, 0.3% Triton X-100 + 10% serum) for 16 hours at 4°C. A biotinylated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Yin Y  et al. Interleukin 7 up-regulates CD95 protein on CD4+ T cells by affecting mRNA alternative splicing: priming for a synergistic effect on HIV-1 reservoir maintenance. J Biol Chem 290:35-45 (2015). WB ; Human . Read more (PubMed: 25411246) »

See 1 Publication for this product

Customer reviews and Q&As

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I would be very happy to raise a discount code for testing ab98056 with ChIP with mouse samples. I would appreciate if you could please provide your customer’s details in order to do it.
As soon as you send me the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Mouse Brain Day 25)
Mouse Brain Day 25
neutral buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Yes - TBS-Tween (0.1%)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 28 2012

I am very pleased to hear you would like to accept our offer and test ab98056 in IHC-P in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P in mouse and include t...

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