Overview

  • Product name

  • Description

    Rabbit polyclonal to Ago1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI) of Human Ago1 (NP_036331).

  • Positive control

    • THP1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

  • Function

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similarities

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization

    Cytoplasm > P-body.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGO1 antibody
    • AGO1_HUMAN antibody
    • Argonaute 1 antibody
    • Argonaute 1 RISC catalytic component antibody
    • Argonaute1 antibody
    • eIF 2C 1 antibody
    • eIF-2C 1 antibody
    • eIF2C 1 antibody
    • EIF2C antibody
    • Eif2c1 antibody
    • Eukaryotic translation initiation factor 2C 1 antibody
    • GERP95 antibody
    • Golgi Endoplasmic Reticulum protein 95 kDa antibody
    • hAgo1 antibody
    • Protein argonaute 1 antibody
    • Protein argonaute-1 antibody
    • Putative RNA binding protein Q99 antibody
    • Putative RNA-binding protein Q99 antibody
    • Q99 antibody
    see all

Images

  • Anti-Ago1 antibody (ab98056) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 97 kDa



    Gel concentration: 6-18%
  • ab98056 staining Ago1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and blocked with 10% serum for 12 hours at 4°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS, 0.3% Triton X-100 + 10% serum) for 16 hours at 4°C. A biotinylated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Yin Y  et al. Interleukin 7 up-regulates CD95 protein on CD4+ T cells by affecting mRNA alternative splicing: priming for a synergistic effect on HIV-1 reservoir maintenance. J Biol Chem 290:35-45 (2015). WB ; Human . Read more (PubMed: 25411246) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for the new lot GR91516-1. The estimated delivery date is xxx. I apologize about the delay.

To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thank you for contacting us.
I would be very happy to raise a discount code for testing ab98056 with ChIP with mouse samples. I would appreciate if you could please provide your customer’s details in order to do it.
As soon as you send me the end user details, I'll send the discount code back.
Thank you very much, and do not hesitate to contact me for further details or information.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Brain Day 25)
Specification
Mouse Brain Day 25
Fixative
neutral buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - TBS-Tween (0.1%)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 28 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab98056 in IHC-P in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P in mouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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