Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-AGO3 antibody
See all AGO3 primary antibodies
DescriptionRabbit polyclonal to AGO3
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide corresponding to Human AGO3 aa 350-450.
(Peptide available as
- This antibody gave a positive signal in rat and mouse dorsal root ganglia tissue lysates in WB.
General notesProtein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).
This product was previously labelled as Argonaute 3 / eIF2C 3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab3593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Cellular localizationCytoplasm > P-body.
- Information by UniProt
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
ab3593 staining AGO3 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at 25°C and then incubated with ab3593 at a 1/50 dilution for 1 hour 30 minutes at 37°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
ab3593 has been referenced in 1 publication.
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529