Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9576] to AGO3
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-AGO3 antibody [EPR9576]
See all AGO3 primary antibodies
DescriptionRabbit monoclonal [EPR9576] to AGO3
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human AGO3.
Database link: Q9H9G7
- WB: HeLa, Daudi, A549 cell lysates. Human fetal brain and fetal kidney lysates.
This product was previously labelled as Argonaute 3 / eIF2C 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
KO cell lysates
Our Abpromise guarantee covers the use of ab154844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 97 kDa.|
FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Cellular localizationCytoplasm > P-body.
- Information by UniProt
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/2000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Argonaute RISC Catalytic Component 3 knockout HeLa lysate
Lane 3 : Daudi lysate
Lane 4 : A549 lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Lanes 1-4: Merged signal (red and green). Green - ab154844 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.
ab154844 Anti-AGO3 antibody [EPR9576] was shown to specifically react with Argonaute RISC Catalytic Component 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265320 (knockout cell lysate ab257819) was used. Wild-type and Argonaute RISC Catalytic Component 3 knockout samples were subjected to SDS-PAGE. ab154844 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µl/ml buffer per lane.
Predicted band size: 97 kDa
ab154844 has been referenced in 1 publication.
- Voutila J et al. Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer. Mol Ther 25:2705-2714 (2017). PubMed: 28882451