Product nameAnti-AGO4 with anti-GAPDH internal loading control antibody
DescriptionRabbit polyclonal to AGO4 with anti-GAPDH internal loading control
Tested applicationsSuitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human AGO4 with anti-GAPDH internal loading control aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This cocktail gave a positive signal in both Human Brain and Testis Tissue lysates as well as the following whole cell lysates: HeLa; A431; Jurkat; HepG2.
This product was previously labelled as Argonaute 4, Argonaute 4 with anti-GAPDH internal loading control
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab94772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa).
ab94772 contains 2 antibodies, generated by raising against an AGO4 immunogen and spiking with anti-GAPDH loading control.
FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Cellular localizationCytoplasm > P-body.
- Information by UniProt
- AGO4_HUMAN antibody
- Argonaute4 antibody
- eIF-2C 4 antibody
All lanes : Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human testis tissue lysate - total protein (ab30257)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37, 97 kDa
Observed band size: 37,97 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The 97 and 37 kDa bands correlate to AGO4 and GAPDH, respectively.
ab94772 has not yet been referenced specifically in any publications.