Overview

  • Product name

    Anti-AGO4 with anti-GAPDH internal loading control antibody
  • Description

    Rabbit polyclonal to AGO4 with anti-GAPDH internal loading control
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human AGO4 with anti-GAPDH internal loading control aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab138813)

  • Positive control

    • This cocktail gave a positive signal in both Human Brain and Testis Tissue lysates as well as the following whole cell lysates: HeLa; A431; Jurkat; HepG2.
  • General notes

     This product was previously labelled as Argonaute 4, Argonaute 4 with anti-GAPDH internal loading control

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa).

ab94772 contains 2 antibodies, generated by raising against an AGO4 immunogen and spiking with anti-GAPDH loading control.

  • Application notes
    Is unsuitable for ChIP,Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
    • Sequence similarities

      Belongs to the argonaute family. Ago subfamily.
      Contains 1 PAZ domain.
      Contains 1 Piwi domain.
    • Cellular localization

      Cytoplasm > P-body.
    • Information by UniProt
    • Database links

    • Alternative names

      • AGO4_HUMAN antibody
      • Argonaute4 antibody
      • eIF-2C 4 antibody
      • eIF2C 4 antibody
      • eif2c4 antibody
      • Eukaryotic translation initiation factor 2C 4 antibody
      • hAgo4 antibody
      • Protein argonaute-4 antibody
      see all

    Images

    • All lanes : Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human brain tissue lysate - total protein (ab29466)
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Human testis tissue lysate - total protein (ab30257)
      Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 37, 97 kDa
      Observed band size: 37,97 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      The 97 and 37 kDa bands correlate to AGO4 and GAPDH, respectively.

    References

    ab94772 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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