Anti-AGPAT1 antibody (ab67018)
Key features and details
- Mouse polyclonal to AGPAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-AGPAT1 antibody
See all AGPAT1 primary antibodies -
Description
Mouse polyclonal to AGPAT1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human AGPAT1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- AGPAT1 transfected 293T cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa). |
Target
-
Function
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. -
Tissue specificity
Widely expressed. Expressed in adipose tissue and at high levels in testis and pancreas. Expressed at lower levels in tissues such as heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte and liver. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. -
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10554 Human
- Omim: 603099 Human
- SwissProt: Q99943 Human
- Unigene: 409230 Human
-
Alternative names
- 1 acyl sn glycerol 3 phosphate acyltransferase alpha antibody
- 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) antibody
- 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) antibody
see all
Images
-
All lanes : Anti-AGPAT1 antibody (ab67018) at 1/500 dilution
Lane 1 : AGPAT1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 32 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (3)
ab67018 has been referenced in 3 publications.
- Lee YJ et al. Aqueous Mulberry Leaf Extract Ameliorates Alcoholic Liver Injury Associating with Upregulation of Ethanol Metabolism and Suppression of Hepatic Lipogenesis. Evid Based Complement Alternat Med 2021:6658422 (2021). PubMed: 34035824
- Lee AT et al. Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice. Antioxidants (Basel) 11:N/A (2021). PubMed: 35052597
- Liu Y et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420