Anti-AGPAT1 antibody (ab67018)
Key features and details
- Mouse polyclonal to AGPAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-AGPAT1 antibody
See all AGPAT1 primary antibodies -
Description
Mouse polyclonal to AGPAT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
AGPAT1 (AAH02402, 1 a.a. ~ 284 a.a) full-length human protein.
-
Positive control
- AGPAT1 transfected 293T cell lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa).
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa). |
Target
-
Relevance
AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 10554 Human
- Omim: 603099 Human
- SwissProt: Q99943 Human
- Unigene: 409230 Human
-
Alternative names
- 1 acyl sn glycerol 3 phosphate acyltransferase alpha antibody
- 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) antibody
- 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) antibody
see all
Images
-
Western blot - Anti-AGPAT1 antibody (ab67018)All lanes : Anti-AGPAT1 antibody (ab67018) at 1/500 dilution
Lane 1 : AGPAT1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 32 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (1)
ab67018 has been referenced in 1 publication.
- Liu Y et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420
