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    agpat1-antibody-ab67018.pdf

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Signal Transduction Metabolism Lipid metabolism
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Anti-AGPAT1 antibody (ab67018)

  • Datasheet
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Abpromise

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Western blot - Anti-AGPAT1 antibody (ab67018)

    Key features and details

    • Mouse polyclonal to AGPAT1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-AGPAT1 antibody
      See all AGPAT1 primary antibodies
    • Description

      Mouse polyclonal to AGPAT1
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      AGPAT1 (AAH02402, 1 a.a. ~ 284 a.a) full-length human protein.

    • Positive control

      • AGPAT1 transfected 293T cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.4
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab67018 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa).

    Target

    • Relevance

      AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
    • Cellular localization

      Membrane; Multi-pass membrane protein.
    • Database links

      • Entrez Gene: 10554 Human
      • Omim: 603099 Human
      • SwissProt: Q99943 Human
      • Unigene: 409230 Human
      • Alternative names

        • 1 acyl sn glycerol 3 phosphate acyltransferase alpha antibody
        • 1 acylglycerol 3 phosphate O acyltransferase 1 (acetoacetly Coenzyme A thiolase) antibody
        • 1 acylglycerol 3 phosphate O acyltransferase 1 (lysophosphatidic acid acyltransferase alpha) antibody
        • 1 acylglycerol 3 phosphate O acyltransferase 1 antibody
        • 1 AGP acyltransferase 1 antibody
        • 1 AGPAT1 antibody
        • AGPAT 1 antibody
        • G15 antibody
        • LPAAT alpha antibody
        • LPAATA antibody
        • Lysophosphatidic acid acyltransferase alpha antibody
        • Lysophospholipid acyltransferase antibody
        • MGC4007 antibody
        • MGC5423 antibody
        • OTTHUMP00000062693 antibody
        • Protein G15 antibody
        see all

      Images

      • Western blot - Anti-AGPAT1 antibody (ab67018)
        Western blot - Anti-AGPAT1 antibody (ab67018)
        All lanes : Anti-AGPAT1 antibody (ab67018) at 1/500 dilution

        Lane 1 : AGPAT1 transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size: 32 kDa
        Observed band size: 27 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab67018? Please let us know so that we can cite the reference in this datasheet.

      ab67018 has been referenced in 1 publication.

      • Liu Y  et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab67018.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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