Anti-AGPAT3 antibody (ab211435)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AGPAT3 antibody
See all AGPAT3 primary antibodies -
Description
Rabbit polyclonal to AGPAT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human AGPAT3 aa 121-170. The exact sequence is proprietary.
Sequence:LAKKELLYVP LIGWTWYFLE IVFCKRKWEE DRDTVVEGLR RLSDYPEYMW
Database link: Q9NRZ7 -
Positive control
- WB - K562 cell lysate IHC-P- Human kidney tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
(without Mg2+, Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab211435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 43 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. -
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 56894 Human
- Entrez Gene: 28169 Mouse
- Entrez Gene: 294324 Rat
- SwissProt: Q9NRZ7 Human
- SwissProt: Q9D517 Mouse
- Unigene: 248785 Human
- Unigene: 141230 Mouse
- Unigene: 100251 Rat
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Alternative names
- 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma antibody
- 1-acylglycerol-3-phosphate O-acyltransferase 3 antibody
- 1-AGP acyltransferase 3 antibody
see all
Images
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Western blot - Anti-AGPAT3 antibody (ab211435)All lanes : Anti-AGPAT3 antibody (ab211435) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate with Synthetic peptide from Human AGPAT3 (aa121-170)
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT3 antibody (ab211435)Immunohistchemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling AGPAT3 with ab211435 at 10 µg/ml.
Datasheets and documents
References
ab211435 has not yet been referenced specifically in any publications.
