Anti-AGPAT3 antibody (ab211435)
Key features and details
- Rabbit polyclonal to AGPAT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGPAT3 antibody
See all AGPAT3 primary antibodies -
Description
Rabbit polyclonal to AGPAT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human AGPAT3 aa 121-170. The exact sequence is proprietary.
Sequence:LAKKELLYVP LIGWTWYFLE IVFCKRKWEE DRDTVVEGLR RLSDYPEYMW
Database link: Q9NRZ7 -
Positive control
- WB - K562 cell lysate IHC-P- Human kidney tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
(without Mg2+, Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab211435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 43 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. -
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 56894 Human
- Entrez Gene: 28169 Mouse
- Entrez Gene: 294324 Rat
- SwissProt: Q9NRZ7 Human
- SwissProt: Q9D517 Mouse
- Unigene: 248785 Human
- Unigene: 141230 Mouse
- Unigene: 100251 Rat
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Alternative names
- 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma antibody
- 1-acylglycerol-3-phosphate O-acyltransferase 3 antibody
- 1-AGP acyltransferase 3 antibody
see all
Images
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Western blot - Anti-AGPAT3 antibody (ab211435)All lanes : Anti-AGPAT3 antibody (ab211435) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate with Synthetic peptide from Human AGPAT3 (aa121-170)
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT3 antibody (ab211435)Immunohistchemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling AGPAT3 with ab211435 at 10 µg/ml.
Protocols
Datasheets and documents
References (0)
ab211435 has not yet been referenced specifically in any publications.
