Overview

  • Product name

  • Description

    Rabbit polyclonal to AGPAT4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human AGPAT4 (internal sequence).

  • Positive control

    • HeLa cell lysate
  • General notes

     This product was previously labelled as 1-AGP acyltransferase 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab135909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.

Target

  • Function

    Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • Pathway

    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • Sequence similarities

    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 acyl sn glycerol 3 phosphate acyltransferase delta antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 4 antibody
    • 1 AGP acyltransferase 4 antibody
    • 1 AGPAT4 antibody
    • 1-acyl-sn-glycerol-3-phosphate acyltransferase delta antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 4 antibody
    • 1-AGP acyltransferase 4 antibody
    • 1-AGPAT 4 antibody
    • AGPAT4 antibody
    • dJ473J16.2 antibody
    • LPAAT delta antibody
    • LPAAT-delta antibody
    • Lysophosphatidic acid acyltransferase delta antibody
    • Lysophosphatidic acid acyltransferase-delta (LPAAT-delta) antibody
    • PLCD_HUMAN antibody
    • RP3 473J16.2 antibody
    see all

Images

  • Anti-AGPAT4 antibody (ab135909) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size: 44 kDa

References

This product has been referenced in:

  • Bradley RM  et al. Lpaatd/Agpat4 deficiency impairs maximal force contractility in soleus and alters fibre type in extensor digitorum longus muscle. Biochim Biophys Acta 1863:700-711 (2018). WB . Read more (PubMed: 29627383) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle depots from C57Bl/6J mice (soleus-EDL-gastr)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Muscle depots from C57Bl/6J mice (soleus-EDL-gastr
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Robin Duncan

Verified customer

Submitted Aug 11 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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