Overview

  • Product name

  • Description

    Rabbit polyclonal to AGPAT7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human AGPAT7 aa 474-524. The exact sequence is proprietary.
    Database link: Q643R3

  • Positive control

    • IP: AGPAT7 IP in HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to AGPAT7 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

8 - 15 µl/mg lysate

Target

  • Relevance

    Members of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction (Ye et al., 2005 [PubMed 16243729]).[supplied by OMIM]
  • Cellular localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • 1 acyl sn glycerol 3 phosphate acyltransferase eta antibody
    • 1 AGP acyltransferase 7 antibody
    • 1 AGPAT 7 antibody
    • Acyltransferase like 3 antibody
    • AGPAT7 antibody
    • AYTL3 antibody
    • LPAAT eta antibody
    • Lpcat4 antibody
    • LPEAT2 antibody
    • Lysophosphatidic acid acyltransferase eta antibody
    • Lysophosphatidylcholine acyltransferase 4 antibody
    see all

Images

  • AGPAT7 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242087 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab242087 at 1/400 dilution.

    Lane 1: ab242087 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

References

ab242087 has not yet been referenced specifically in any publications.

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