Key features and details
- Rabbit polyclonal to AGPAT7
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AGPAT7 antibody
See all AGPAT7 primary antibodies
DescriptionRabbit polyclonal to AGPAT7
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human AGPAT7 aa 474-524. The exact sequence is proprietary.
Database link: Q643R3
- IP: AGPAT7 IP in HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to AGPAT7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 - 15 µl/mg lysate
RelevanceMembers of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 188.8.131.52) family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction (Ye et al., 2005 [PubMed 16243729]).[supplied by OMIM]
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein
- 1 acyl sn glycerol 3 phosphate acyltransferase eta antibody
- 1 AGP acyltransferase 7 antibody
- 1 AGPAT 7 antibody
AGPAT7 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242087 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab242087 at 1/400 dilution.
Lane 1: ab242087 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242087 has not yet been referenced specifically in any publications.