Key features and details
- Rabbit polyclonal to AGPS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AGPS antibody
See all AGPS primary antibodies
DescriptionRabbit polyclonal to AGPS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig
- WB: HepG2 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: NIH/3T3 cells.
This product was previously labelled as Alkyl-DHAP synthase
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 73 kDa.|
|IHC-P||1/500 - 1/1000.|
|ICC/IF||1/50 - 1/200.|
PathwayGlycerolipid metabolism; ether lipid biosynthesis.
Involvement in diseaseDefects in AGPS are the cause of rhizomelic chondrodysplasia punctata type 3 (RCDP3) [MIM:600121]. RCDP3 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.
Sequence similaritiesBelongs to the FAD-binding oxidoreductase/transferase type 4 family.
Contains 1 FAD-binding PCMH-type domain.
Cellular localizationPeroxisome membrane. Localized to the inner aspect of the peroxisomal membrane.
- Information by UniProt
- AAG5 antibody
- ADAP-S antibody
- ADAS antibody
Anti-AGPS antibody (ab236621) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 kDa
Paraffin-embedded human pancreatic cancer tissue stained for AGPS with ab236621 at a 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for AGPS using ab236621 at a dilution of 1/166 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236621 has not yet been referenced specifically in any publications.