Recombinant Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AGR2 + AGR3 antibody [EPR20164-222]
See all AGR2 + AGR3 primary antibodies -
Description
Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human AGR2 His-tagged recombinant protein (aa21-175); Human AGR3 GST-tagged recombinant protein (aa21-166); MCF7, HT-29 and PC-3 whole cell lysates; Mouse and rat stomach lysates; Human lung and stomach lysates. ICC/IF: MCF7 cells. Flow Cyt (intra): MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20164-222 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 20, 19 kDa).
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ICC/IF |
1/500.
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Flow Cyt (Intra) |
1/500.
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Notes |
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WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 20, 19 kDa). |
ICC/IF
1/500. |
Flow Cyt (Intra)
1/500. |
Target
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Relevance
AGR2 (Anterior Gradient 2), also known as AG2 (hAG2, HAG2 in human), or GOB4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 10551 Human
- Entrez Gene: 155465 Human
- Entrez Gene: 23795 Mouse
- Entrez Gene: 403205 Mouse
- Entrez Gene: 298959 Rat
- Entrez Gene: 298961 Rat
- Omim: 606358 Human
- Omim: 609482 Human
see all -
Alternative names
- AG 2 antibody
- AG 3 antibody
- AG2 antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line.
Negative control: HeLa (PMID: 16551856).
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/10000 dilution
Lane 1 : Human AGR2 His-tagged recombinant protein (aa21-175)
Lane 2 : Human AGR3 GST-tagged recombinant protein (aa21-166)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20, 19 kDa
Observed band size: 17,42 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 seconds.
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All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20, 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
According to the literature AGR2 is not expressed in HeLa (PMID: 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.
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All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Rat stomach tissue lysate
Lane 3 : Human lung tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20, 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/2000 dilution + Human stomach lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20, 19 kDa
Observed band size: 17,18 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab216071 has been referenced in 1 publication.
- Wrenn SM et al. Avian lungs: A novel scaffold for lung bioengineering. PLoS One 13:e0198956 (2018). PubMed: 29949597