Overview

  • Product name

    Anti-AGR2 + AGR3 antibody [EPR20164-222]
    See all AGR2 + AGR3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human AGR2 + AGR3 aa 1 to the C-terminus. The exact sequence is proprietary. from AGR2
    Database link: O95994

  • Positive control

    • WB: Human AGR2 His-tagged recombinant protein (aa21-175); Human AGR3 GST-tagged recombinant protein (aa21-166); MCF7, HT-29 and PC-3 whole cell lysates; Mouse and rat stomach lysates; Human lung and stomach lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20164-222
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 20, 19 kDa).
ICC/IF 1/500.
Flow Cyt 1/500.

Target

  • Relevance

    AGR2 (Anterior Gradient 2), also known as AG2 (hAG2, HAG2 in human), or GOB4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • AG 2 antibody
    • AG 3 antibody
    • AG2 antibody
    • AG3 antibody
    • Anterior gradient 2 antibody
    • Anterior gradient 3 antibody
    • Anterior gradient 3 homolog antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient homolog 3 antibody
    • Anterior gradient protein 2 homolog antibody
    • Anterior gradient protein 2 homolog precursor antibody
    • Anterior gradient protein 3 homolog antibody
    • Anterior gradient protein 3 homolog precursor antibody
    • BCMP11 antibody
    • Breast cancer membrane protein 11 antibody
    • Epididymis secretory protein Li 116 antibody
    • GOB 4 antibody
    • HAG 2 antibody
    • hAG 3 antibody
    • hAG3 antibody
    • HEL S 116 antibody
    • HPC8 antibody
    • PDIA17 antibody
    • PDIA18 antibody
    • Protein disulfide isomerase family A, member 17 antibody
    • Protein disulfide isomerase family A, member 18 antibody
    • Secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • XAG 2 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line.

    Negative control: HeLa (PMID: 16551856).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/10000 dilution

    Lane 1 : Human AGR2 His-tagged recombinant protein (aa21-175)
    Lane 2 : Human AGR3 GST-tagged recombinant protein (aa21-166)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 seconds.

  • All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    According to the literature AGR2 is not expressed in HeLa (PMID: 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.

  • All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : Mouse stomach lysate
    Lane 2 : Rat stomach lysate
    Lane 3 : Human lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/2000 dilution + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,18 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

References

This product has been referenced in:

See 1 Publication for this product

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