RecombinantRabMAb

Recombinant Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)

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Immunocytochemistry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
  • Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free
    See all AGR2 + AGR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20164-222] to AGR2 + AGR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251553 is the carrier-free version of ab216071. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251553 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 20, 19 kDa).
Notes
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 20, 19 kDa).

Target

  • Relevance

    AGR2 (Anterior Gradient 2), also known as AG2 (hAG2, HAG2 in human), or GOB4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.

  • Cellular localization

    Secreted

  • Database links

    see all

  • Alternative names

    • AG 2 antibody
    • AG 3 antibody
    • AG2 antibody
    • AG3 antibody
    • Anterior gradient 2 antibody
    • Anterior gradient 3 antibody
    • Anterior gradient 3 homolog antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient homolog 3 antibody
    • Anterior gradient protein 2 homolog antibody
    • Anterior gradient protein 2 homolog precursor antibody
    • Anterior gradient protein 3 homolog antibody
    • Anterior gradient protein 3 homolog precursor antibody
    • BCMP11 antibody
    • Breast cancer membrane protein 11 antibody
    • Epididymis secretory protein Li 116 antibody
    • GOB 4 antibody
    • HAG 2 antibody
    • hAG 3 antibody
    • hAG3 antibody
    • HEL S 116 antibody
    • HPC8 antibody
    • PDIA17 antibody
    • PDIA18 antibody
    • Protein disulfide isomerase family A, member 17 antibody
    • Protein disulfide isomerase family A, member 18 antibody
    • Secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • XAG 2 antibody
    see all

Images

  • Immunocytochemistry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Immunocytochemistry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)

    This data was developed using ab216071, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. Negative control: HeLa (PMID: 16551856). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/10000 dilution

    Lane 1 : Human AGR2 His-tagged recombinant protein (aa21-175)
    Lane 2 : Human AGR3 GST-tagged recombinant protein (aa21-166)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,42 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab216071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lane 2: 3 seconds.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab216071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    According to the literature AGR2 is not expressed in HeLa (PMID: 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    All lanes : Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : Rat stomach tissue lysate
    Lane 3 : Human lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab216071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/2000 dilution + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20, 19 kDa
    Observed band size: 17,18 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab216071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    This data was developed using ab216071, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Flow Cytometry - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    This data was developed using ab216071, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
    Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251553? Please let us know so that we can cite the reference in this datasheet.

ab251553 has not yet been referenced specifically in any publications.

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