Dapi was used to stain the nuclei.
Negative picture is attached in this abreview.
We also tested fixation 5min methanol (-20%) followed by permeabilization but it also does not work.
In addition, we tested an antibody from another supplier which worked fine with both fixation methods. We can send a picture if needed.
We are currently investigating this issue and hope to come to a resolution based on our Abpromise guarantee.
Dr. Steffen Rickelt
Submitted Nov 12 2013
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