Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Agrin antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer & Tris pH 9.0
Sample
Mouse Tissue sections (mouse kidney)
Specification
mouse kidney
Permeabilization
Yes - 0.2 % Triton X in PBS
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight in PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500

Additional data

Additional Notes
Dapi was used to stain the nuclei. Negative picture is attached in this abreview. We also tested fixation 5min methanol (-20%) followed by permeabilization but it also does not work. In addition, we tested an antibody from another supplier which worked fine with both fixation methods. We can send a picture if needed.
Abcam response
We are currently investigating this issue and hope to come to a resolution based on our Abpromise guarantee.

Dr. Steffen Rickelt

Verified customer

Submitted Nov 12 2013

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