Western blot abreview for Anti-Agrin antibody

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Purified protein (proteoglycan preparation from human milk)
Specification
proteoglycan preparation from human milk
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 78 kDa
Exposure
30 second(s)
Positive control
sample containing Agrin as proven by proteomic analysis

Dr. Serena Leone

Verified customer

Submitted Dec 04 2013

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