Key features and details
- Guinea pig polyclonal to AGRP
- Suitable for: IHC-Fr
- Reacts with: Rat, Sheep
Product nameAnti-AGRP antibody
See all AGRP primary antibodies
DescriptionGuinea pig polyclonal to AGRP
Host speciesGuinea pig
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Rat, Sheep
Predicted to work with: Mouse, Cow, Human, Pig
- IHC-Fr: Sheep hypothalamus tissue; rat brain tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20ºC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab228495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/1000 - 1/2000.
Permeabilization suggested: 0.1% Triton X-100 in blocking buffer.
FunctionPlays a role in weight homeostasis. Plays a role in the central control of feeding. Reduces food intake. Inhibits cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Acts primarily on MC3R and MC4R. Has very low activity with MC5R.
Tissue specificityExpressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
Involvement in diseaseGenetic variations in AGRP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesContains 1 agouti domain.
DomainThe presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
Cellular localizationSecreted. Golgi apparatus lumen.
- Information by UniProt
- Agouti related neuropeptide antibody
- Agouti Related Protein Homolog antibody
- Agouti-related protein antibody
4% paraformaldehyde-fixed, frozen section of sheep hypothalamus tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with a biotinylated secondary antibody.
4% paraformaldehyde-fixed, frozen section of rat brain tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with Alexa Fluor® 568 Goat anti-Guinea pig at 1/500 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228495 has been referenced in 1 publication.
- Prezotto LD et al. Influences of maternal nutrient restriction and arginine supplementation on visceral metabolism and hypothalamic circuitry of offspring. Domest Anim Endocrinol 65:71-79 (2018). PubMed: 30007131