Overview

  • Product name

  • Description

    Rabbit polyclonal to AGS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AGS3 aa 9-84.
    Sequence:

    IERHRVHLRSATLRDAVPATLHLLPCEVAVDGPAPVGRFFTPAIRQGPEG LEVSFRGRCLRGEEVAVPPGLVGYVM


    Database link: Q8TDP1

  • Positive control

    • WB: U-251 MG cell lysate. ICC/IF: MCF7 cells.
  • General notes

     This product was previously labelled as RNase H2 subunit C

     

Properties

Applications

Our Abpromise guarantee covers the use of ab221596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Relevance

    The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AGS3 antibody
    • Aicardi-Goutieres syndrome 3 protein antibody
    • AYP1 antibody
    • Ribonuclease H2 subunit C antibody
    • Ribonuclease HI subunit C antibody
    • RNase H1 small subunit antibody
    • RNASEH2C antibody
    • RNH2C antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for AGS3 (green) using ab221596 at 4 µg/ml in ICC/IF. 

  • All lanes : Anti-AGS3 antibody (ab221596) at 1/500 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 18 kDa

References

ab221596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221596.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up