Key features and details
- Rabbit polyclonal to AGS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AGS3 antibody
See all AGS3 primary antibodies
DescriptionRabbit polyclonal to AGS3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human AGS3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2.
This product was previously labelled as RNase H2 subunit C
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).|
RelevanceThe RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.
- AGS3 antibody
- Aicardi-Goutieres syndrome 3 protein antibody
- AYP1 antibody
All lanes : Anti-AGS3 antibody (ab80880) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
AGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).
ab80880 has not yet been referenced specifically in any publications.