Overview

  • Product name

  • Description

    Rabbit polyclonal to AGS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AGS3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90249)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2.
  • General notes

     This product was previously labelled as RNase H2 subunit C

     

Properties

Applications

Our Abpromise guarantee covers the use of ab80880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

Target

  • Relevance

    The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AGS3 antibody
    • Aicardi-Goutieres syndrome 3 protein antibody
    • AYP1 antibody
    • Ribonuclease H2 subunit C antibody
    • Ribonuclease HI subunit C antibody
    • RNase H1 small subunit antibody
    • RNASEH2C antibody
    • RNH2C antibody
    see all

Images

  • All lanes : Anti-AGS3 antibody (ab80880) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    AGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).

References

ab80880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up