Recombinant Anti-AGXT antibody [EPR13232-59] (ab178699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13232-59] to AGXT
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AGXT antibody [EPR13232-59]
See all AGXT primary antibodies -
Description
Rabbit monoclonal [EPR13232-59] to AGXT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human AGXT aa 100-250. The exact sequence is proprietary.
Database link: P21549 -
Positive control
- HepG2 and Human fetal liver lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13232-59 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). |
Target
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Tissue specificity
Liver. -
Involvement in disease
Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. -
Sequence similarities
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix. - Information by UniProt
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Database links
- Entrez Gene: 189 Human
- Entrez Gene: 11611 Mouse
- Entrez Gene: 24792 Rat
- Omim: 604285 Human
- SwissProt: P21549 Human
- SwissProt: O35423 Mouse
- SwissProt: P09139 Rat
- Unigene: 144567 Human
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Alternative names
- AGT antibody
- AGT1 antibody
- Agxt antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab178699 has been referenced in 2 publications.
- Sanchez-Baltasar R et al. Efficient and Fast Generation of Relevant Disease Mouse Models by In Vitro and In Vivo Gene Editing of Zygotes. CRISPR J 5:422-434 (2022). PubMed: 35686982
- Sauer V et al. Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-Like Cells Using Stem Cell Technology. Cell Transplant 25:2221-2243 (2016). PubMed: 27512979