Overview

  • Product name

    Anti-AGXT antibody [EPR13232]
    See all AGXT primary antibodies
  • Description

    Rabbit monoclonal [EPR13232] to AGXT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human AGXT.
    Database link: P21549

  • Positive control

    • HepG2 and Human fetal liver lysates; Human hepatocellular carcinoma and Human liver tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Tissue specificity

      Liver.
    • Involvement in disease

      Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.
    • Sequence similarities

      Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
    • Cellular localization

      Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • AGT antibody
      • AGT1 antibody
      • Agxt antibody
      • AGXT1 antibody
      • Alanine glyoxylate aminotransferase antibody
      • Alanine glyoxylate aminotransferase3 antibody
      • Alanine--glyoxylate aminotransferase antibody
      • EC 2.6.1.44 antibody
      • EC 2.6.1.51 antibody
      • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
      • Hepatic peroxisomal alanine:glyoxylate aminotransferase antibody
      • L alanine glyoxylate aminotransferase 1 antibody
      • MS773 antibody
      • PH1 antibody
      • Serine pyruvate aminotransferase antibody
      • Serine--pyruvate aminotransferase antibody
      • Serine--pyruvate aminotransferase, mitochondrial antibody
      • Serine:pyruvate aminotransferase antibody
      • SPAT antibody
      • SPT antibody
      • SPYA_HUMAN antibody
      • TLH6 antibody
      see all

    Images

    • All lanes : Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa

    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with ab178708 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab178708 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Western blot analysis of Mouse liver (20μg) labelling AGXT with ab178708 at 1/2000 in 5% NFDM /TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (ab97051) (1/20000) was used as the secondary antibody.

    References

    ab178708 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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