Overview

  • Product name

  • Description

    Rabbit polyclonal to AGXT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AGXT2 aa 196-328.
    Database link: Q9BYV1

  • Positive control

    • WB: LO2 whole cell lysate. IHC-P: Human liver and kidney tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.
IHC-P 1/500 - 1/1000.
WB 1/500 - 1/5000.

Target

  • Relevance

    AGXT2 is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • (R)-3-amino-2-methylpropionate--pyruvate transaminase antibody
    • AGT2 antibody
    • AI303810 antibody
    • AI663818 antibody
    • Alanine glyoxylate aminotransferase 2 antibody
    • Alanine glyoxylate aminotransferase 2 mitochondrial antibody
    • Beta-ALAAT II antibody
    • Beta-alanine-pyruvate aminotransferase antibody
    • DAIBAT antibody
    see all

Images

  • Anti-AGXT2 antibody (ab237622) at 1/500 dilution + LO2 (human immortal liver cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human kidney tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human liver tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AGXT2 using ab237622 at a dilution of 1/233 in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

References

ab237622 has not yet been referenced specifically in any publications.

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