Anti-AHA1 antibody [25F2.D9] (ab91259)

Overview

  • Product name
    Anti-AHA1 antibody [25F2.D9]
    See all AHA1 primary antibodies
  • Description
    Rat monoclonal [25F2.D9] to AHA1
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Mouse AHA1

  • Positive control
    • ICC/IF: HeLa cells. IHC-P: Mouse back skin. IP: HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    25F2.D9
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • Function
    Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Tissue specificity
    Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • Sequence similarities
    Belongs to the AHA1 family.
  • Cellular localization
    Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
    • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
    • AHA 1 antibody
    • AHA1 antibody
    • AHSA 1 antibody
    • Ahsa1 antibody
    • AHSA1_HUMAN antibody
    • C14orf3 antibody
    • HSPC322 antibody
    • p38 antibody
    see all

Images

  • ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
  • Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate

    Predicted band size: 38 kDa

  • Paraffin-embedded mouse back skin tissue stained for AHA1 using ab91259 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is FITC Goat Anti-Rat.

  • ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .

References

ab91259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these ...

Read More

Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (https://...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up