Overview

  • Product name

    Anti-AHA1 antibody [25F2.D9]
    See all AHA1 primary antibodies
  • Description

    Rat monoclonal [25F2.D9] to AHA1
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IP, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Mouse AHA1

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Mouse back skin. IP: HeLa cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    25F2.D9
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • Function

    Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Tissue specificity

    Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • Sequence similarities

    Belongs to the AHA1 family.
  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
    • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
    • AHA 1 antibody
    • AHA1 antibody
    • AHSA 1 antibody
    • Ahsa1 antibody
    • AHSA1_HUMAN antibody
    • C14orf3 antibody
    • HSPC322 antibody
    • p38 antibody
    see all

Images

  • Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate

    Predicted band size: 38 kDa

  • ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
  • Paraffin-embedded mouse back skin tissue stained for AHA1 using ab91259 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is FITC Goat Anti-Rat.

  • ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .

References

ab91259 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these proteins and zebrafish is 60% whereas the residues 1-31 have a homology of 100%. However, the epitope has not been mapped and we do not know where the antibody will bind the protein. Only ab64998 was raised against an internal peptide sequence. I am in touch with the lab to check what region had been selected and to check homology. We usually say that a homology of 85% or higher has a good chance of cross reactivity. With 60% homology I am not sure. If the epitope happens to be in the N-terminal region however, the likelihood of binding to the zebrafish protein would be very high, though. I will be in touch as soon as I have more information about ab64998. Please let me know if you would like to try one of the above antibodies. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (https://www.abcam.com/Hsp90-antibody-16F1-ab13494.html) - it would be guaranteed for this species as well as the listed tested applications. For Aha1, we do not have an antibody tested in this species. But if you please could send me the protein sequence for zebrafish, I'd be happy to check homology and to see whether any of the Aha1 antibodies can be used for this species. If the homology is about 85% or higher, you can use our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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