Overview

  • Product name

  • Description

    Rabbit polyclonal to AHA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human AHA1 (internal sequence). The exact sequence is proprietary.
    Database link: O95433

  • Positive control

    • WB: HeLa cell extract; Jurkat whole cell lysate. IHC-P: A549 xenograft tissue. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/30000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • Tissue specificity

    Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • Sequence similarities

    Belongs to the AHA1 family.
  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
    • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
    • AHA 1 antibody
    • AHA1 antibody
    • AHSA 1 antibody
    • Ahsa1 antibody
    • AHSA1_HUMAN antibody
    • C14orf3 antibody
    • HSPC322 antibody
    • p38 antibody
    see all

Images

  • All lanes : Anti-AHA1 antibody (ab228492) at 1/30000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : AHA1 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 38 kDa

  • All lanes : Anti-AHA1 antibody (ab228492) at 1/30000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : AHA1 knockout HeLa cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    10% SDS-PAGE

  • Paraffin-embedded A549 xenograft tissue stained for AHA1 with ab228492 at 1:100 dilution in immunohistochemical analysis.

  • Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for AHA1 (green) using ab228492 at 1:500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Anti-AHA1 antibody (ab228492) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    10% SDS-PAGE

References

ab228492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228492.
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