RecombinantRabMAb

Recombinant Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)

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Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
  • Immunoprecipitation - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
  • Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
  • Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13888] to AHA1 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AHA1 antibody [EPR13888] - C-terminal
    See all AHA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13888] to AHA1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AHA1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O95433

  • Positive control

    • RAW 264.7, PC12, NIH 3T3, HeLa, HepG2 and A549 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13888

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.

  • Tissue specificity

    Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.

  • Sequence similarities

    Belongs to the AHA1 family.

  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
      • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
      • AHA 1 antibody
      • AHA1 antibody
      • AHSA 1 antibody
      • Ahsa1 antibody
      • AHSA1_HUMAN antibody
      • C14orf3 antibody
      • HSPC322 antibody
      • p38 antibody
      see all

    Images

    • Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      All lanes : Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 38 kDa

    • Immunoprecipitation - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      Immunoprecipitation - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)

      Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab191432 at 1/30 dilution (lane 1) or PBS control (lane 2).
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      Western blot - Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      All lanes : Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432) at 1/1000 dilution

      Lane 1 : RAW 264.7 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 38 kDa

    • Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)
      Anti-AHA1 antibody [EPR13888] - C-terminal (ab191432)

    References (0)

    Publishing research using ab191432? Please let us know so that we can cite the reference in this datasheet.

    ab191432 has not yet been referenced specifically in any publications.

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