Overview

  • Product name

    Anti-AHAS antibody [EPR12843]
    See all AHAS primary antibodies
  • Description

    Rabbit monoclonal [EPR12843] to AHAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AHAS.
    Database link: A1L0T0

  • Positive control

    • HeLa, HepG2 and A549 cell lysates. Permeabilized HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as ILVBL

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12843
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Tissue specificity

      Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.
    • Sequence similarities

      Belongs to the TPP enzyme family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 209L8 antibody
      • Acetolactate synthase homolog antibody
      • Acetolactate synthase like protein antibody
      • acetolactate synthase, bacterial, homolog of antibody
      • Acetolactate synthase-like protein antibody
      • AHAS antibody
      • FLJ39061 antibody
      • ILV2H antibody
      • IlvB (bacterial acetolactate synthase) like antibody
      • IlvB like protein antibody
      • IlvB-like protein antibody
      • Ilvbl antibody
      • ILVBL_HUMAN antibody
      • MGC1269 antibody
      • MGC19535 antibody
      see all

    Images

    • All lanes : Anti-AHAS antibody [EPR12843] (ab177157) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 67 kDa

    • Flow cytometric analysis of permeabilized Hela cells labeling AHAS with ab177157 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    References

    ab177157 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177157.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up