Recombinant Anti-AHAS antibody [EPR12843] (ab177157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12843] to AHAS
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AHAS antibody [EPR12843]
See all AHAS primary antibodies -
Description
Rabbit monoclonal [EPR12843] to AHAS -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human AHAS.
Database link: A1L0T0 -
Positive control
- HeLa, HepG2 and A549 cell lysates. Permeabilized HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12843 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Expressed in all tissues tested, with highest expression in heart, pancreas and placenta. -
Sequence similarities
Belongs to the TPP enzyme family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10994 Human
- Omim: 605770 Human
- SwissProt: A1L0T0 Human
- Unigene: 78880 Human
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Alternative names
- 209L8 antibody
- Acetolactate synthase homolog antibody
- Acetolactate synthase like protein antibody
see all
Images
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All lanes : Anti-AHAS antibody [EPR12843] (ab177157) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa -
Intracellular flow cytometric analysis of permeabilized Hela cells labeling AHAS with ab177157 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab177157 has not yet been referenced specifically in any publications.