Key features and details
- Rabbit polyclonal to AHNAK
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AHNAK antibody
See all AHNAK primary antibodies
DescriptionRabbit polyclonal to AHNAK
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human AHNAK aa 100-200 conjugated to keyhole limpet haemocyanin.
Database link: Q09666
- This antibody gave a positive signal in JEG3 whole cell lysates within IF/ICC.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab135137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.
FunctionMay be required for neuronal cell differentiation.
Sequence similaritiesContains 1 PDZ (DHR) domain.
- Information by UniProt
- AHNAK antibody
- AHNAK nucleoprotein (desmoyokin) antibody
- AHNAK nucleoprotein antibody
ICC/IF image of ab135137 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab135137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The expression profile observed is consistent with what has been described in the literature (PMID:7698224, PMID:7769263 and PMID:11535620).
ab135137 has not yet been referenced specifically in any publications.