Key features and details
- Mouse monoclonal [EM-09] to AHNAK
- Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-AHNAK antibody [EM-09]
See all AHNAK primary antibodies
DescriptionMouse monoclonal [EM-09] to AHNAK
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Does not react with: Rat
Recombinant fragment corresponding to Human AHNAK (N terminal).
Database link: Q09666
- WB: 1% lauryl maltoside lysate of HeLa cells. ICC/IF: Human primary fibroblasts. HeLa cells. IHC-Fr: Mouse tongue tissue. Flow Cyt: HeLa cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesab68556 is purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab68556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 629 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
Permeabilization is required.
FunctionMay be required for neuronal cell differentiation.
Sequence similaritiesContains 1 PDZ (DHR) domain.
- Information by UniProt
- AHNAK antibody
- AHNAK nucleoprotein (desmoyokin) antibody
- AHNAK nucleoprotein antibody
Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/ml
Performed under non-reducing conditions.
Predicted band size: 629 kDa
The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting: 30 mA, 90 min. Detection antibody concentration: 2 microgram/ml. Please note the conditions were not optimalized.
Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
ab68556 at 1/250 dilution staining AHNAK (red) in HeLa cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).
Ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).
Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab68556 has been referenced in 3 publications.
- Zhang Z et al. Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition. J Cancer 10:3860-3870 (2019). PubMed: 31333803
- Johnson S et al. Mass spectrometry analysis reveals differences in the host cell protein species found in pseudotyped lentiviral vectors. Biologicals 52:59-66 (2018). WB . PubMed: 29361371
- Park JW et al. AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development. Mol Cancer Res 16:1287-1298 (2018). PubMed: 29724814