Overview

  • Product name

  • Description

    Rabbit polyclonal to AHRR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (PQSEPPHQLC ARGRGEQSCT CRAAEAAPVV KREPLDSPQW ATHSQGMVPG) of Human AHRR (NP_065782)

  • Positive control

    • HepG2 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
  • Tissue specificity

    Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly in the nuclear compartment. First cytoplasmic, translocates into the nuclear compartment upon interaction with ARNT in the cytoplasmic compartment.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHH antibody
    • AhR repressor antibody
    • AhRR antibody
    • AHRR_HUMAN antibody
    • aryl hydrocarbon hydroxylase regulator antibody
    • aryl hydrocarbon receptor regulator antibody
    • Aryl hydrocarbon receptor repressor antibody
    • Arylhydrocarbon receptor repressor antibody
    • bHLHe77 antibody
    • Class E basic helix-loop-helix protein 77 antibody
    • Dioxin receptor repressor antibody
    • KIAA1234 antibody
    • MGC167813 antibody
    • MGC176630 antibody
    • OTTHUMP00000221488 antibody
    • OTTHUMP00000221489 antibody
    see all

Images

  • Anti-AHRR antibody (ab85666) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 92 kDa
    why is the actual band size different from the predicted?



    Gel concentration 12%

References

This product has been referenced in:

  • Ma Y  et al. Ferulic acid ameliorates nonalcoholic fatty liver disease and modulates the gut microbiota composition in high-fat diet fed ApoE-/- mice. Biomed Pharmacother 113:108753 (2019). Read more (PubMed: 30856537) »
  • Joo MS  et al. miR-125b transcriptionally increased by Nrf2 inhibits AhR repressor, which protects kidney from cisplatin-induced injury. Cell Death Dis 4:e899 (2013). Read more (PubMed: 24176857) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (HepG2, 1: cytoplasmic fraction, 2: nuclear fractio)
Gel Running Conditions
Reduced Denaturing (10 % SDS Gel)
Loading amount
50 µg
Specification
HepG2, 1: cytoplasmic fraction, 2: nuclear fractio
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 01 2019

Answer

Thank you for getting back to me and for passing some further information. I could offer either a new vial free of charge or a credit note which can be used for future purchase. Please could you get back to your troubled customer and discuss how he/she would like to proceed with this case. I look forward to hearing from you so

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Question

LOT NUMBER gr54067-1 ORDER NUMBER 937542 DESCRIPTION OF THE PROBLEM No signal or weak signal and no band in positive control (HepG2 cell lysate) SAMPLE •Species: human •What’s cell line or tissue SK-Hep1 •Cell extract or Nuclear extract: total protein cell lysate •Purified protein or Recombinant protein: no PRIMARY ANTIBODY •Species: Rabbit •Reacts against: •At what dilution(s) have you tested this antibody: 1 ug/ml •What dilution buffer was used: 5% no fat milk in TBS •Incubation time: overnight •Incubation temperature: 4℃ •What washing steps were done: 5 min/time with TBST (0.1% tritonX20), 5 times DETECTION METHOD ECL+ POSITIVE AND NEGATIVE CONTROLS USED Yes. (50 μg HepG2 cell lysate) ANTIBODY STORAGE CONDITIONS 937542 SAMPLE PREPARATION •What lysis buffer was used: RIPA buffer •What protease inhibitors were used: Aprotenin, Leupeptin, PMSF •What loading buffer was used: β-ME •Phosphatase inhibitors: β-glycerophosphate, Na3VO4 •Did you heat the samples: temperature and time: 95℃ 5min AMOUNT OF PROTEIN LOADED 50 μg ELECTROPHORESIS/GEL CONDITIONS •Reducing or non reducing gel: non reducing gel •Reducing agent: •Gel percentage : 10% SDS-PAGE TRANSFER AND BLOCKING CONDITIONS •Transfer conditions: (Type of membrane, Protein transfer verified): PVDF, transfer condition: 4℃ 300 mA 2hr •Buffer: TBS •Blocking agent: milk, BSA, serum, what percentage: 5% no fat milk •Incubation time: 1 hr •Incubation temperature: RT SECONDARY ANTIBODY •Species:goat •Reacts against: rabbit •At what dilution(s) have you tested this antibody: 1:3000 •Incubation time: 2 hr •Wash steps: 5 min/time with TBST (0.1% tritonX20), 5 times •Fluorochrome or enzyme conjugate: enzyme conjugate •Do you know whether the problems you are experiencing come from the secondary? No HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? protein loading

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Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. ANTIBODY STORAGE CONDITIONS 937542 Questions: - Could you please clarify how this product was stored and shipped to the end-user? - Does the detection system work fine? Have you used it successfully with another primary antibody? I look forward to hearing from you and hope to solve this problem as soon as possible.

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