Overview

  • Product name

  • Description

    Rabbit polyclonal to AHSA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Human AHSA2.
    Database link: Q719I0

  • Positive control

    • ICC/IF: Heat shocked HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab240877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/80. optimal dilutions for assays should be determined by the user

Target

Images

  • Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling AHSA2 using ab240877 at 1/80 for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 minutes at RT.

    Secondary Antibody: FITC Goat Anti-Rabbit IgG (green) at 1/200 for 2 hours at RT.

    The nuclear counterstain was DAPI (blue) at 1/40000 for 2 hours at RT.

    (A) DAPI (blue) nuclear stain (B) ab240877 (C) Composite.

    Heat shocked at 42°C for 1 hour.

  • Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling AHSA2 using ab240877 at 1/80 dilution for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 minutes at RT.

    Secondary Antibody: R-PE Goat Anti-Rabbit IgG (yellow) at 1/200 for 2 hours at RT.

    The nuclear Counterstain was DAPI (blue) at 1/40000 for 2 hours at RT.

    (A) DAPI (blue) nuclear stain (B) ab240877 (C) Composite.

    Heat shocked at 42°C for 1 hour.

References

ab240877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240877.
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