Anti-AHSG antibody (ab47979)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AHSG antibody
See all AHSG primary antibodies -
Description
Rabbit polyclonal to AHSG -
Host species
Rabbit -
Tested applications
Suitable for: RIA, WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human Alpha 2 HS Glycoprotein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| RIA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 40 kDa. | |
| ELISA | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Function
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
Tissue specificity
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
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Alternative names
- 59 kDa bone sialic acid-containing protein antibody
- A2HS antibody
- Aa2-066 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AHSG antibody (ab47979)ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab47979 has not yet been referenced specifically in any publications.
