Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab47979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RIA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionPromotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Tissue specificitySynthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Sequence similaritiesBelongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- 59 kDa bone sialic acid-containing protein antibody
- A2HS antibody
- Aa2-066 antibody
ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab47979 has not yet been referenced specifically in any publications.