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Cardiovascular Blood Serum Proteins
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Anti-AHSG antibody (ab47979)

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Immunocytochemistry/ Immunofluorescence - Anti-AHSG antibody (ab47979)

    Key features and details

    • Rabbit polyclonal to AHSG
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human AHSG protein (ab156750)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-AHSG antibody
      See all AHSG primary antibodies
    • Description

      Rabbit polyclonal to AHSG
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Human Alpha 2 HS Glycoprotein

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.50
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Metabolism
      • Types of disease
      • Obesity

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human AHSG protein (ab156750)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab47979 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
    • Tissue specificity

      Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
    • Sequence similarities

      Belongs to the fetuin family.
      Contains 2 cystatin fetuin-A-type domains.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02765 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 197 Human
      • Omim: 138680 Human
      • SwissProt: P02765 Human
      • Unigene: 324746 Human
      • Alternative names

        • 59 kDa bone sialic acid-containing protein antibody
        • A2HS antibody
        • Aa2-066 antibody
        • AHS antibody
        • Ahsg alpha-2-HS-glycoprotein antibody
        • Ahsg antibody
        • Alpha 2 HS Glycoprotein antibody
        • Alpha 2 Z globulin antibody
        • Alpha-2-HS-glycoprotein antibody
        • Alpha-2-HS-glycoprotein chain B antibody
        • Alpha-2-Z-globulin antibody
        • Asialofetuin antibody
        • Ba alpha 2 glycoprotein antibody
        • Ba-alpha-2-glycoprotein antibody
        • BSP antibody
        • Countertrypin antibody
        • Fetua antibody
        • FETUA_HUMAN antibody
        • Fetuin , mouse, homolog of antibody
        • Fetuin A antibody
        • Fetuin-A antibody
        • Glycoprotein PP63 antibody
        • HSGA antibody
        • pp63 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-AHSG antibody (ab47979)
        Immunocytochemistry/ Immunofluorescence - Anti-AHSG antibody (ab47979)
        ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab47979? Please let us know so that we can cite the reference in this datasheet.

      ab47979 has not yet been referenced specifically in any publications.

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