Anti-Ahsp antibody (ab231040)
Key features and details
- Rabbit polyclonal to Ahsp
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Ahsp antibody
See all Ahsp primary antibodies -
Description
Rabbit polyclonal to Ahsp -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Rat Ahsp aa 1 to the C-terminus. (N-terminal His-tag and GST-tag. Expressed in E.coli).
Database link: A9UMW3 -
Positive control
- WB: Recombinant rat Ahsp protein. Rat and mouse liver lysates. IHC-P: Rat stomach, liver and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231040 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 12 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 12 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. -
Tissue specificity
Expressed in blood and bone marrow. -
Sequence similarities
Belongs to the AHSP family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 170812 Mouse
- Entrez Gene: 293522 Rat
- SwissProt: Q9CY02 Mouse
- Unigene: 423023 Mouse
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Alternative names
- AHSP antibody
- AHSP_HUMAN antibody
- Alpha hemoglobin stabilizing protein antibody
see all
Images
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All lanes : Anti-Ahsp antibody (ab231040) at 3 µg/ml
Lane 1 : Rat liver lysate
Lane 2 : Mouse liver lysate
Secondary
All lanes : HRP-linked Guinea pig Anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 12 kDa -
Paraffin-embedded rat stomach tissue stained for Ahsp with ab231040 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Ahsp antibody (ab231040) at 3 µg/ml + Recombinant rat Ahsp protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 12 kDa -
Paraffin-embedded rat kidney tissue stained for Ahsp with ab231040 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded rat liver tissue stained for Ahsp with ab231040 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231040 has not yet been referenced specifically in any publications.