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    ahsp-antibody-ab231040.pdf

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Signal Transduction Protein Trafficking Chaperones Other Chaperones
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Anti-Ahsp antibody (ab231040)

  • Datasheet
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Abpromise

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Find out more.

Western blot - Anti-Ahsp antibody (ab231040)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)
  • Western blot - Anti-Ahsp antibody (ab231040)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)

Key features and details

  • Rabbit polyclonal to Ahsp
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human Ahsp protein (ab87752)

View more associated products

Overview

  • Product name

    Anti-Ahsp antibody
    See all Ahsp primary antibodies
  • Description

    Rabbit polyclonal to Ahsp
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Rat Ahsp aa 2-102. (N-terminal His-tag and GST-tag. Expressed in E.coli).
    Sequence:

    APFQSNKDLISTGIKEFNVLLDQQVFDDPLMPEEDMKTVVNDWVNLYINY YKKHVLGEQQEQDGALKELQQELSTLGSQFLAKYRIILKSKETPSSKPPS S


    Database link: A9UMW3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant rat Ahsp protein. Rat and mouse liver lysates. IHC-P: Rat stomach, liver and kidney tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab231040 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Erythrocytic Lineage
    • Cardiovascular
    • Blood
    • Erythrocytes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
  • Recombinant Protein

    • Recombinant Human Ahsp protein (ab87752)

Applications

Our Abpromise guarantee covers the use of ab231040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.
  • Tissue specificity

    Expressed in blood and bone marrow.
  • Sequence similarities

    Belongs to the AHSP family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9NZD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 170812 Mouse
    • Entrez Gene: 293522 Rat
    • SwissProt: Q9CY02 Mouse
    • Unigene: 423023 Mouse
    • Alternative names

      • AHSP antibody
      • AHSP_HUMAN antibody
      • Alpha hemoglobin stabilizing protein antibody
      • Alpha-hemoglobin-stabilizing protein antibody
      • EDRF antibody
      • ERAF antibody
      • Erythroid associated factor antibody
      • Erythroid differentiation associated factor antibody
      • Erythroid differentiation related factor antibody
      • Erythroid differentiation-related factor antibody
      • Erythroid-associated factor antibody
      see all

    Images

    • Western blot - Anti-Ahsp antibody (ab231040)
      Western blot - Anti-Ahsp antibody (ab231040)
      All lanes : Anti-Ahsp antibody (ab231040) at 3 µg/ml

      Lane 1 : Rat liver lysate
      Lane 2 : Mouse liver lysate

      Secondary
      All lanes : HRP-linked Guinea pig Anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 12 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)

      Paraffin-embedded rat stomach tissue stained for Ahsp with ab231040 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-Ahsp antibody (ab231040)
      Western blot - Anti-Ahsp antibody (ab231040)
      Anti-Ahsp antibody (ab231040) at 3 µg/ml + Recombinant rat Ahsp protein

      Secondary
      HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution

      Predicted band size: 12 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)

      Paraffin-embedded rat kidney tissue stained for Ahsp with ab231040 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (ab231040)

      Paraffin-embedded rat liver tissue stained for Ahsp with ab231040 at 10 µg/ml in immunohistochemical analysis. DAB staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab231040? Please let us know so that we can cite the reference in this datasheet.

    ab231040 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab231040.
    Please use the links above to contact us or submit feedback about this product.

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