Overview

  • Product name

    Anti-Ahsp antibody [EPR12319]
    See all Ahsp primary antibodies
  • Description

    Rabbit monoclonal [EPR12319] to Ahsp
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ahsp aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NZD4

  • Positive control

    • Human fetal liver lysate; Human fetal spleen tissue, Human lung tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 12 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.
    • Tissue specificity

      Expressed in blood and bone marrow.
    • Sequence similarities

      Belongs to the AHSP family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AHSP antibody
      • AHSP_HUMAN antibody
      • Alpha hemoglobin stabilizing protein antibody
      • Alpha-hemoglobin-stabilizing protein antibody
      • EDRF antibody
      • ERAF antibody
      • Erythroid associated factor antibody
      • Erythroid differentiation associated factor antibody
      • Erythroid differentiation related factor antibody
      • Erythroid differentiation-related factor antibody
      • Erythroid-associated factor antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ahsp with ab180861 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Ahsp antibody [EPR12319] (ab180861) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Predicted band size: 12 kDa

    • Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab180861 at 1/10 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human fetal spleen tissue labeling Ahsp with ab180861 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab180861 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (fetal liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH9, 100C, 20min
    Permeabilization
    No
    Specification
    fetal liver
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 14 2015

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