Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human AIBZIP peptide (ab22911).|
FunctionTranscriptional activator that may play a role in the unfolded protein response. Binds to the UPR element (UPRE) but not to CRE element. Preferentially binds DNA with to the consensus sequence 5'-T[GT]ACGT[GA][GT]-3' and has transcriptional activation activity from UPRE. Binds to NF-kappa-B site and has transcriptional activation activity from NF-kappa-B-containing regulatory elements.
Tissue specificityAccording to PubMed:11830526, exclusively expressed in the prostate. Expressed in breast and prostate cancer cell lines. Expressed in prostatic luminal epithelial cells (at protein level). Expression is significantly more abundant in prostate cancer than in benign prostatic tissue (prostatic hyperplasia). According to PubMed:12111373, also expressed in brain, pancreas and skeletal muscle, and at lower levels in small intestine, testis, leukocyte and thymus.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
modificationsN-glycosylated in the C-terminal region.
Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2). PS1 cleavage may be suppressed by a determinant in the C-terminal region.
Cellular localizationNucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. May also be located in Golgi apparatus.
- Information by UniProt
- 1700012K17Rik antibody
- AIbZIP antibody
- Androgen induced basic leucine zipper antibody
Anti-AIBZIP antibody (ab2474) at 0.5 µg/ml + Human placental lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
ab2474 at a concentration of 0.5
µg/ml staining approximately 50kDa AIBZIP in human placental lysate (RIPA buffer, total of 35 µg per lane) by Western blot (ECL).
ab2474 at 2.5ug/ml staining AIBZIP in human prostate tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab2474 has not yet been referenced specifically in any publications.