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  1. Link

    aicar-acadesineaica-riboside-ampk-activator-ab120358.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases
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AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (1)References (14)

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Chemical Structure - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
  • Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
  • Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)

Key features and details

  • Cell-permeable activator of AMP-activated protein kinase
  • CAS Number: 2627-69-2
  • Soluble in water to 50 mM and in DMSO to 100 mM (with heating)
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    AICAR (Acadesine/AICA riboside), AMPK activator
  • Description

    Cell-permeable activator of AMP-activated protein kinase
  • Alternative names

    • Acadesine
    • AICA riboside
  • Biological description

    Cell-permeable activator of AMP-activated protein kinase. Is taken up into cells by adenosine transporters and phosphorylated by adenosine kinase to the active nucleotide ZMP (5-aminoimidazole-4-carboxamide ribonucleoside), which mimics effects of AMP on the AMPK system. Active in vivo and in vitro.

    Also available in simple stock solutions (ab146713) - add 1 ml of water to get an exact, ready-to-use concentration.

  • CAS Number

    2627-69-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    5-Amino-1-[(2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl]-1H-imidazole-4-carboxamide
  • Molecular weight

    258.23
  • Molecular formula

    C9H14N4O5
  • PubChem identifier

    17513
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 50 mM and in DMSO to 100 mM (with heating)
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Refer to SDS for further information

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    NC(=O)c2ncn([C@@H]1O[C@H](CO)[C@H](O)[C@@H]1O)c2N
  • Source

    Synthetic

  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Biochemicals
    • Product Range
    • Just Add Water
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Kinase
    • AMP-kinase
    • Activators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    Chemical Structure - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    2D chemical structure image of ab120358, AICAR (Acadesine/AICA riboside), AMPK activator
  • Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    ab39400 staining AMPK alpha 1 (phospho S496) in HepG2 cells treated with AICAR (ab120358), by ICC/IF. Increase in AMPK alpha 1 (phospho S496) expression correlates with increased concentration of AICAR, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120358 (AICAR) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab39400 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    T24 (transitional cell carcinoma) and UMUC-3 cells were pre-incubated with G9a activator AICAR (1mM, ab120358) for 4 h and then treated with BIX-01294 (1.5 μM) for 24h. The levels of p-AMPKα, p-mTOR and LC-3 I/II was examined.

    Credit: Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390

     

     

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (14)

Publishing research using ab120358? Please let us know so that we can cite the reference in this datasheet.

ab120358 has been referenced in 14 publications.

  • Sternlieb T  et al. An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi. PLoS Negl Trop Dis 15:e0009435 (2021). PubMed: 34029334
  • Gao P  et al. Inhibition of Mitochondrial Calcium Overload by SIRT3 Prevents Obesity- or Age-Related Whitening of Brown Adipose Tissue. Diabetes 69:165-180 (2020). PubMed: 31712319
  • Gong Y  et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169
  • Hawley SA  et al. Mechanism of Activation of AMPK by Cordycepin. Cell Chem Biol 27:214-222.e4 (2020). PubMed: 31991096
  • Ji R  et al. Electric field down-regulates CD9 to promote keratinocytes migration through AMPK pathway. Int J Med Sci 17:865-873 (2020). PubMed: 32308539
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

I'd like to know how much would it last 250mg Aicar or what's the recommended usage? Thank you.

Read More

Abcam community

Verified customer

Asked on Sep 05 2012

Answer

Thank you for contacting us.
This AICAR can be stored unopened for up to 6 months at -20C under dessicating conditions. After opening, we recommend using immediately for best results. You can store aliquots in tightly sealed vials at -20C for up to one month after opening.
This product is for research purposes only, and more information about its use can be found in the following references:
Towler MC & Hardie DG AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res 100:328-41 (2007). PubMed: 17307971
Gadalla AE et al. AICA riboside both activates AMP-activated protein kinase and competes with adenosine for the nucleoside transporter in the CA1 region of the rat hippocampus. J Neurochem 88:1272-82 (2004). PubMed: 15009683
Guigas B et al. Beyond AICA riboside: in search of new specific AMP-activated protein kinase activators. IUBMB Life 61:18-26 (2009). PubMed: 18798311
I hope this helps, please let me know if you need any additional information or assistance.

Read More

Abcam Scientific Support

Answered on Sep 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

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