AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)

Submit a review Q&A (1)References (4)

Overview

  • Product name

    AICAR (Acadesine/AICA riboside), AMPK activator

  • Description

    Cell-permeable activator of AMP-activated protein kinase

  • Alternative names

    • Acadesine
    • AICA riboside

  • Biological description

    Cell-permeable activator of AMP-activated protein kinase. Is taken up into cells by adenosine transporters and phosphorylated by adenosine kinase to the active nucleotide ZMP (5-aminoimidazole-4-carboxamide ribonucleoside), which mimics effects of AMP on the AMPK system. Active in vivo and in vitro.

    Also available in simple stock solutions (ab146713) - add 1 ml of water to get an exact, ready-to-use concentration.

  • Purity

    > 98%

  • CAS Number

    2627-69-2

  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    ab39400 staining AMPK alpha 1 (phospho S496) in HepG2 cells treated with AICAR (ab120358), by ICC/IF. Increase in AMPK alpha 1 (phospho S496) expression correlates with increased concentration of AICAR, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120358 (AICAR) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab39400 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
    Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    T24 (transitional cell carcinoma) and UMUC-3 cells were pre-incubated with G9a activator AICAR (1mM, ab120358) for 4 h and then treated with BIX-01294 (1.5 μM) for 24h. The levels of p-AMPKα, p-mTOR and LC-3 I/II was examined.

    Credit: Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390

     

     

References

This product has been referenced in:

  • Guigas B  et al. Beyond AICA riboside: in search of new specific AMP-activated protein kinase activators. IUBMB Life 61:18-26 (2009). Read more (PubMed: 18798311) »
  • Towler MC & Hardie DG AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res 100:328-41 (2007). Read more (PubMed: 17307971) »
  • Gadalla AE  et al. AICA riboside both activates AMP-activated protein kinase and competes with adenosine for the nucleoside transporter in the CA1 region of the rat hippocampus. J Neurochem 88:1272-82 (2004). Read more (PubMed: 15009683) »
See all 4 Publications for this product

Publishing research using ab120358? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I'd like to know how much would it last 250mg Aicar or what's the recommended usage? Thank you.

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Answer

Thank you for contacting us.
This AICAR can be stored unopened for up to 6 months at -20C under dessicating conditions. After opening, we recommend using immediately for best results. You can store aliquots in tightly sealed vials at -20C for up to one month after opening.
This product is for research purposes only, and more information about its use can be found in the following references:
Towler MC & Hardie DG AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res 100:328-41 (2007). PubMed: 17307971
Gadalla AE et al. AICA riboside both activates AMP-activated protein kinase and competes with adenosine for the nucleoside transporter in the CA1 region of the rat hippocampus. J Neurochem 88:1272-82 (2004). PubMed: 15009683
Guigas B et al. Beyond AICA riboside: in search of new specific AMP-activated protein kinase activators. IUBMB Life 61:18-26 (2009). PubMed: 18798311
I hope this helps, please let me know if you need any additional information or assistance.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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