AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)
Overview
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Product name
AICAR (Acadesine/AICA riboside), AMPK activator -
Description
Cell-permeable activator of AMP-activated protein kinase -
Alternative names
- Acadesine
- AICA riboside
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Biological description
Cell-permeable activator of AMP-activated protein kinase. Is taken up into cells by adenosine transporters and phosphorylated by adenosine kinase to the active nucleotide ZMP (5-aminoimidazole-4-carboxamide ribonucleoside), which mimics effects of AMP on the AMPK system. Active in vivo and in vitro.
Also available in simple stock solutions (ab146713) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 98% -
CAS Number
2627-69-2 -
Chemical structure
Properties
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Chemical name
5-Amino-1-[(2R,3S,4R,5R)-tetrahydro-3,4-dihydroxy-5-(hydroxymethyl)furan-2-yl]-1H-imidazole-4-carboxamide -
Molecular weight
258.23 -
Molecular formula
C9H14N4O5 -
PubChem identifier
17513 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 50 mM and in DMSO to 100 mM (with heating) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(=O)c2ncn([C@@H]1O[C@H](CO)[C@H](O)[C@@H]1O)c2N -
Source
Synthetic
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)ab39400 staining AMPK alpha 1 (phospho S496) in HepG2 cells treated with AICAR (ab120358), by ICC/IF. Increase in AMPK alpha 1 (phospho S496) expression correlates with increased concentration of AICAR, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120358 (AICAR) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab39400 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - AICAR (Acadesine/AICA riboside), AMPK activator (ab120358)Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/T24 (transitional cell carcinoma) and UMUC-3 cells were pre-incubated with G9a activator AICAR (1mM, ab120358) for 4 h and then treated with BIX-01294 (1.5 μM) for 24h. The levels of p-AMPKα, p-mTOR and LC-3 I/II was examined.
Credit: Li F et al. PLoS One. 2015; 10(9): e0138390. doi: 10.1371/journal.pone.0138390
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (8)
ab120358 has been referenced in 8 publications.
- Gong Y et al. Metformin Inhibits Tumor Metastasis through Suppressing Hsp90a Secretion in an AMPKa1-PKC? Dependent Manner. Cells 9:N/A (2020). PubMed: 31936169
- Hawley SA et al. Mechanism of Activation of AMPK by Cordycepin. Cell Chem Biol 27:214-222.e4 (2020). PubMed: 31991096
- Ji R et al. Electric field down-regulates CD9 to promote keratinocytes migration through AMPK pathway. Int J Med Sci 17:865-873 (2020). PubMed: 32308539
- Tong X et al. Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy. Cell Prolif 53:e12714 (2020). PubMed: 31696568
- Morishita M et al. AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway. Int J Oncol 50:23-30 (2017). PubMed: 27878239
- Li F et al. G9a Inhibition Induces Autophagic Cell Death via AMPK/mTOR Pathway in Bladder Transitional Cell Carcinoma. PLoS One 10:e0138390 (2015). PubMed: 26397365
- Khan M et al. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy. BMC Neurosci 16:42 (2015). PubMed: 26174015
- van Dam AD et al. Salsalate activates brown adipose tissue in mice. Diabetes 64:1544-54 (2015). PubMed: 25475439
