Anti-AICDA antibody (ab59361)
Key features and details
- Rabbit polyclonal to AICDA
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AICDA antibody
See all AICDA primary antibodies -
Description
Rabbit polyclonal to AICDA -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to 14 amino acids near the C-terminus of Human AID
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Human
|
IHC-P |
Human
|
WB |
Human
|
All applications |
Mouse
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
|
ICC |
Use a concentration of 10 µg/ml.
|
Notes |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. -
Tissue specificity
Strongly expressed in lymph nodes and tonsils. -
Involvement in disease
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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Database links
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
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Alternative names
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
see all
Images
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All lanes : Anti-AICDA antibody (ab59361) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Ramos cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 24 kDa
Observed band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody (ab59361)ab59361 (4 µg/ml) staining AICDA in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ab59361 at 10µg/ml, staining human AID in Ramos cells
Protocols
Datasheets and documents
References (9)
ab59361 has been referenced in 9 publications.
- Jiao J et al. AID assists DNMT1 to attenuate BCL6 expression through DNA methylation in diffuse large B-cell lymphoma cell lines. Neoplasia 22:142-153 (2020). PubMed: 32062068
- Gion Y et al. Up-regulation of activation-induced cytidine deaminase and its strong expression in extra-germinal centres in IgG4-related disease. Sci Rep 9:761 (2019). PubMed: 30679751
- Araki A et al. IL-21 Enhances the Development of Colitis-Associated Colon Cancer: Possible Involvement of Activation-Induced Cytidine Deaminase Expression. J Immunol 202:3326-3333 (2019). PubMed: 31019062
- Jiao J et al. AID and TET2 co-operation modulates FANCA expression by active demethylation in diffuse large B cell lymphoma. Clin Exp Immunol 195:190-201 (2019). PubMed: 30357811
- Karpurapu M et al. Krüppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation. PLoS One 9:e93362 (2014). WB, ChIP ; Mouse . PubMed: 24695324
- Geisberger R et al. Lysine residue at position 22 of the AID protein regulates its class switch activity. PLoS One 7:e30667 (2012). WB . PubMed: 22363466
- Häsler J et al. Cytoplasmic activation-induced cytidine deaminase (AID) exists in stoichiometric complex with translation elongation factor 1a (eEF1A). Proc Natl Acad Sci U S A 108:18366-71 (2011). WB ; Human . PubMed: 22042842
- Wang M et al. Altering the spectrum of immunoglobulin V gene somatic hypermutation by modifying the active site of AID. J Exp Med 207:141-53, S1-6 (2010). WB . PubMed: 20048284
- Sylvestre EL et al. Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells. Cell Reprogram 12:519-28 (2010). WB ; Human . PubMed: 20936903