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    aicda-antibody-ab59361.pdf

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Immunology Immunoglobulins Class Switching
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Anti-AICDA antibody (ab59361)

  • Datasheet
Reviews (3)Q&A (5)References (9)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-AICDA antibody (ab59361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody (ab59361)
  • Immunocytochemistry - Anti-AICDA antibody (ab59361)

Key features and details

  • Rabbit polyclonal to AICDA
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human AICDA protein (denatured) (ab139251)
Primary
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Anti-Histone H4 (mono methyl K20) antibody [EPR16999] - ChIP Grade (ab177188)

View more associated products

Overview

  • Product name

    Anti-AICDA antibody
    See all AICDA primary antibodies
  • Description

    Rabbit polyclonal to AICDA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids near the C-terminus of Human AID

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Class Switching
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human AICDA protein (denatured) (ab139251)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab59361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC
Use a concentration of 10 µg/ml.
Notes
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC
Use a concentration of 10 µg/ml.

Target

  • Function

    RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • Tissue specificity

    Strongly expressed in lymph nodes and tonsils.
  • Involvement in disease

    Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • Sequence similarities

    Belongs to the cytidine and deoxycytidylate deaminase family.
  • Target information above from: UniProt accession Q9GZX7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57379 Human
    • Entrez Gene: 11628 Mouse
    • Omim: 605257 Human
    • SwissProt: Q9GZX7 Human
    • SwissProt: Q9WVE0 Mouse
    • Unigene: 149342 Human
    • Unigene: 391503 Mouse
    • Alternative names

      • Activation induced cytidine deaminase antibody
      • Activation induced deaminase antibody
      • Activation-induced cytidine deaminase antibody
      • AICDA antibody
      • AICDA_HUMAN antibody
      • AID antibody
      • ARP 2 antibody
      • ARP2 antibody
      • CDA 2 antibody
      • CDA2 antibody
      • Cytidine aminohydrolase antibody
      • HIGM2 antibody
      • Integrated into Burkitt's lymphoma cell line Ramos antibody
      see all

    Images

    • Western blot - Anti-AICDA antibody (ab59361)
      Western blot - Anti-AICDA antibody (ab59361)
      All lanes : Anti-AICDA antibody (ab59361) at 2 µg/ml

      Lane 1 : Ramos cell lysate
      Lane 2 : Ramos cell lysate with immunizing peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 24 kDa
      Observed band size: 24 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody (ab59361)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody (ab59361)
      ab59361 (4 µg/ml) staining AICDA in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
      Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Immunocytochemistry - Anti-AICDA antibody (ab59361)
      Immunocytochemistry - Anti-AICDA antibody (ab59361)
      ab59361 at 10µg/ml, staining human AID in Ramos cells

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab59361? Please let us know so that we can cite the reference in this datasheet.

    ab59361 has been referenced in 9 publications.

    • Jiao J  et al. AID assists DNMT1 to attenuate BCL6 expression through DNA methylation in diffuse large B-cell lymphoma cell lines. Neoplasia 22:142-153 (2020). PubMed: 32062068
    • Gion Y  et al. Up-regulation of activation-induced cytidine deaminase and its strong expression in extra-germinal centres in IgG4-related disease. Sci Rep 9:761 (2019). PubMed: 30679751
    • Araki A  et al. IL-21 Enhances the Development of Colitis-Associated Colon Cancer: Possible Involvement of Activation-Induced Cytidine Deaminase Expression. J Immunol 202:3326-3333 (2019). PubMed: 31019062
    • Jiao J  et al. AID and TET2 co-operation modulates FANCA expression by active demethylation in diffuse large B cell lymphoma. Clin Exp Immunol 195:190-201 (2019). PubMed: 30357811
    • Karpurapu M  et al. Krüppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation. PLoS One 9:e93362 (2014). WB, ChIP ; Mouse . PubMed: 24695324
    • Geisberger R  et al. Lysine residue at position 22 of the AID protein regulates its class switch activity. PLoS One 7:e30667 (2012). WB . PubMed: 22363466
    • Häsler J  et al. Cytoplasmic activation-induced cytidine deaminase (AID) exists in stoichiometric complex with translation elongation factor 1a (eEF1A). Proc Natl Acad Sci U S A 108:18366-71 (2011). WB ; Human . PubMed: 22042842
    • Wang M  et al. Altering the spectrum of immunoglobulin V gene somatic hypermutation by modifying the active site of AID. J Exp Med 207:141-53, S1-6 (2010). WB . PubMed: 20048284
    • Sylvestre EL  et al. Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells. Cell Reprogram 12:519-28 (2010). WB ; Human . PubMed: 20936903

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-AICDA antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B cell line)
    Loading amount
    30 µg
    Specification
    B cell line
    Gel Running Conditions
    Reduced Denaturing (14% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Dr. Yiming Zhong

    Verified customer

    Submitted Dec 20 2011

    Question

    Yes, I am interested in testing ab59361 in mouse.  Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2011

    Answer

    DISCOUNT CODE: XXXXXXXX Expiration date: [30/03/2012] I am very pleased to hear you would like to accept our offer and test ab59361 in Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    Answered on Nov 29 2011

    Question

    Good morning, I am a postdoc at the Ohio State University.  I would like to test the anti-AID antibody (ab59361) in mouse. Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 28 2011

    Answer

    Thank you very much for your interest in ab59361. To our knowledge, ab59361 has not been tested in Mouse (predicted species only). Therefore, I can offer a discount off a future purchase if you buy ab59361 now, test it in Mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab59361 in Mouse. I will then send a discount code. This code must be issued before purchasing ab59361 so please wait for my reply before ordering. 2. Purchase ab59361 either by phone, fax, or online (www.abcam.com). 3. Test it in Mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab59361 turns out to be unsuitable for Mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    Answered on Nov 28 2011

    ChIP abreview for Anti-AICDA antibody - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - whole cell (Splenic B lymphocytes)
    Specification
    Splenic B lymphocytes
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 5 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    Recruitment at Switch mu region
    anti-H4 antibody ChIP
    Negative control
    aid-/- cell extracts
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 24 2011

    Question

    I am interested in acquiring the AID antibody (ab59361) and testing it on our our NanoPro CB000 immunoassay system. As discussed I am emailing you such that I can receive the discount voucher code to order this product.  regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 09 2011

    Answer

    DISCOUNT CODE:XXXXXXXX Expiration date: <09-01-2012> I am very pleased to hear you would like to accept our offer and test ab59361 with "mouse". This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2011

    Question

    Customer interested in buying ab59361 however asked if we can provide free samples. Could you also let us know if you have antibodies against T-cell or B-cell rearrangements?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2011

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews We do have range of antibodies available against T cell and B cell receptor alpha, beta and gamma chain. To search these please type "TCR" or BCR in the search box provided on our website; system then will actually give you sorted product results. You can then tailor the specific antibodies according to your requirements using criteria given on Abcam website. Please click the below given links for more information: https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=tcr https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=bcr I hope this information is helpful. Please do not hesitate to contact us again with any other questions. PS: Let me know if you would like to test the antibody with mouse sample I will then send you a discount code; which will give you next product free of charge in exchange of an Abreview.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2011

    Question

    I would like to know more about the abreview program.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2011

    Answer

    Thank you very much for your interest in ab59361. To our knowledge, ab59361 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab59361 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab59361 in mouse. I will then send a discount code. This code must be issued before purchasing ab59361 so please wait for my reply before ordering. 2. Purchase ab59361either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab59361 turns out to be unsuitable for mouse you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2011

    ChIP abreview for Anti-AICDA antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Primary human B cell chromatin)
    Specification
    Primary human B cell chromatin
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 4 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Taqman
    Positive control
    IgM enhancer and switch region
    Negative control
    epsilon globin and NeuroD1
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 30 2011

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