Product nameAnti-AICDA antibody
See all AICDA primary antibodies
DescriptionRabbit polyclonal to AICDA
Tested applicationsSuitable for: ChIP, IHC-P, WB, ICCmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to 14 amino acids near the C-terminus of Human AID
- Ramos cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab59361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|ICC||Use a concentration of 10 µg/ml.|
FunctionRNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
Tissue specificityStrongly expressed in lymph nodes and tonsils.
Involvement in diseaseDefects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.
- Information by UniProt
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
All lanes : Anti-AICDA antibody (ab59361) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Ramos cell lysate with immunizing peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 24 kDa
Observed band size: 24 kDa
ab59361 (4 µg/ml) staining AICDA in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab59361 at 10µg/ml, staining human AID in Ramos cells
This product has been referenced in:
- Gion Y et al. Up-regulation of activation-induced cytidine deaminase and its strong expression in extra-germinal centres in IgG4-related disease. Sci Rep 9:761 (2019). Read more (PubMed: 30679751) »
- Araki A et al. IL-21 Enhances the Development of Colitis-Associated Colon Cancer: Possible Involvement of Activation-Induced Cytidine Deaminase Expression. J Immunol 202:3326-3333 (2019). Read more (PubMed: 31019062) »