Overview

  • Product name

    Anti-AICDA antibody - Aminoterminal end
    See all AICDA primary antibodies
  • Description

    Rabbit polyclonal to AICDA - Aminoterminal end
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the N terminal amino acids 1-60 of Human AICDA (Uniprot ID Q9GZX7).

  • Positive control

    • Raji, 293T whole cell lysate, HeLa cells, Cal27 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • Tissue specificity

    Strongly expressed in lymph nodes and tonsils.
  • Involvement in disease

    Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • Sequence similarities

    Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation induced cytidine deaminase antibody
    • Activation induced deaminase antibody
    • Activation-induced cytidine deaminase antibody
    • AICDA antibody
    • AICDA_HUMAN antibody
    • AID antibody
    • ARP 2 antibody
    • ARP2 antibody
    • CDA 2 antibody
    • CDA2 antibody
    • Cytidine aminohydrolase antibody
    • HIGM2 antibody
    • Integrated into Burkitt's lymphoma cell line Ramos antibody
    see all

Images

  • Anti-AICDA antibody - Aminoterminal end (ab137397) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab137397 at 1/500 dilution. Costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelled with ab137397 at 1/500 dilution.

References

ab137397 has not yet been referenced specifically in any publications.

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