Recombinant
RabMAb

Recombinant Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22061] to AICL - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-AICL antibody [EPR22061] - BSA and Azide free
    See all AICL primary antibodies

  • Description

    Rabbit monoclonal [EPR22061] to AICL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human AICL aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92478

  • Positive control

    • IHC-P: Human spleen tissue.

  • General notes

    ab235626 is the carrier-free version of ab221158 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab235626 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CLEC2B

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab235626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance

    This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell activation antigen. An alternative splice variant has been described but its full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [Entrez Gene]

  • Cellular localization

    Membrane; Single-pass type II membrane protein

  • Database links

    • Alternative names

      • Activation induced C type lectin antibody
      • AICL antibody
      • C type lectin domain family 2 member B antibody
      • C type lectin superfamily member 2 antibody
      • CLEC2 antibody
      • CLECSF2 antibody
      • HP10085 antibody
      • IFN alpha2b inducing related protein 1 antibody
      • IFNRG1 antibody
      see all

    Images

    • Immunoprecipitation - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)
      Immunoprecipitation - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)

      AICL was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab221158 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221158 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: U937 whole cell lysate 10 µg (Input). 

      Lane 2: ab221158 IP in U937 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221158 in U937 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      The expression profile observed is due to glycosylation as described in the literature (PMID: 23929856).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)

      Immunohistochemical analysis of paraffin-embedded human non-Hodgkin’s lymphoma tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human non-Hodgkin’s lymphoma (PMID: 23929856). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)
      Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling AICL with ab221158 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic and membranous staining in U937 cell line.

      Negative control: HeLa (PMID: 23929856).

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (ab235626)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human spleen (PMID: 9038101; PMID: 23929856). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab235626? Please let us know so that we can cite the reference in this datasheet.

    ab235626 has not yet been referenced specifically in any publications.

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