Key features and details
- Rabbit polyclonal to AIDA
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AIDA antibody
See all AIDA primary antibodies
DescriptionRabbit polyclonal to AIDA
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cynomolgus monkey, Orangutan
- IHC-P: Human cerebellum tissue. ICC/IF: A431 cells. WB: U-251 MG cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab251782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.|
FunctionActs as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
Tissue specificityWidely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
Sequence similaritiesBelongs to the AIDA family.
- Information by UniProt
- 2610208M17Rik antibody
- AIDA antibody
- AIDA_HUMAN antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for AIDA (green) using ab251782 at 4 µg/ml in ICC/IF.
Paraffin-embedded human cerebellum tissue stained for AIDA using ab251782 at 1/150 dilution in immunohistochemical analysis.
All lanes : Anti-AIDA antibody (ab251782) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Predicted band size: 35 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab251782 has not yet been referenced specifically in any publications.