Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)

Overview

  • Product name

    Anti-AIDA antibody [EPR19161] - BSA and Azide free
    See all AIDA primary antibodies
  • Description

    Rabbit monoclonal [EPR19161] to AIDA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse AIDA aa 1-300. The exact sequence is proprietary.
    Database link: Q8C4Q6

  • Positive control

    • WB: Immortalized wild type MEF cell lysate.
  • General notes

    Ab224718 is the carrier-free version of ab199323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab224718 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19161
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 35 kDa).

Under our experimental conditions ab199323 detected non-specific bands in WB. However, the band of interest at ∼36kDa disappears from the knockout sample demonstrating the specificity of the antibody.

Target

  • Function

    Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
  • Tissue specificity

    Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
  • Sequence similarities

    Belongs to the AIDA family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610208M17Rik antibody
    • AIDA antibody
    • AIDA_HUMAN antibody
    • Axin interaction partner and dorsalization antagonist antibody
    • Axin interactor antibody
    • Axin interactor dorsalization associated protein antibody
    • Axin interactor, dorsalization associated antibody
    • C1orf80 antibody
    • Chromosome 1 open reading frame 80 antibody
    • dorsalization-associated protein antibody
    • RP11-378J18.7 antibody
    • UPF0491 protein C1orf80 antibody
    see all

Images

  • All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

    Lane 1 : Immortalized wild type MEF (mouse embryonic fibroblast cell line), whole cell lysate
    Lane 2 : Aida knockout immortalized MEF, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.

    The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199323).

  • All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

    Lane 1 : Wild type mouse lung homogenate
    Lane 2 : Aida knockout mouse lung homogenate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.

    The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199323).

References

ab224718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224718.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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