Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20903-53] to AIDA - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-AIDA antibody [EPR20903-53] - BSA and Azide free
See all AIDA primary antibodies
DescriptionRabbit monoclonal [EPR20903-53] to AIDA - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Mouse AIDA aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q8C4Q6
- WB: MEF, NIH/3T3, RAE 264.7, C2C12, HeLa, U-87 MG, C6 and PC-12 whole cell lysates.
ab274421 is the carrier-free version of ab234419. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab274421 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab274421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).|
FunctionActs as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
Tissue specificityWidely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
Sequence similaritiesBelongs to the AIDA family.
- Information by UniProt
- 2610208M17Rik antibody
- AIDA antibody
- AIDA_HUMAN antibody
All lanes : Anti-AIDA antibody [EPR20903-53] (ab234419) at 1/1000 dilution
Lane 1 : Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : AIDA knockout MEF whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 6 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 8 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 9 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
This data was developed using ab234419, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. ab234419 and ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
AIDA knockout MEF cell lysate was kindly provided by our collaborator.
Exposure time: 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab274421 has not yet been referenced specifically in any publications.