Overview

  • Product name
    Anti-AIF antibody - C-terminal
    See all AIF primary antibodies
  • Description
    Goat polyclonal to AIF - C-terminal
  • Host species
    Goat
  • Specificity
    ab205370 is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human AIF aa 602-613 (C terminal) (Cysteine residue).
    Sequence:

    NEVAKLFNIHED


    Database link: O95831

  • Positive control
    • WB: Jurkat and NIH3T3 cell lysates and mouse heart, pig heart, rat heart, mouse kidney and rat kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab205370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).

Target

  • Function
    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
  • Involvement in disease
    Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
  • Sequence similarities
    Belongs to the FAD-dependent oxidoreductase family.
  • Post-translational
    modifications
    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
  • Cellular localization
    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIFM1 antibody
    • AIFM1_HUMAN antibody
    • Apoptosis inducing factor 1, mitochondrial antibody
    • Apoptosis inducing factor antibody
    • Apoptosis inducing factor, mitochondrion associated, 1 antibody
    • Apoptosis-inducing factor 1 antibody
    • CMTX4 antibody
    • COWCK antibody
    • COXPD6 antibody
    • Harlequin antibody
    • Hq antibody
    • mAIF antibody
    • MGC111425 antibody
    • MGC5706 antibody
    • mitochondrial antibody
    • Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
    • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
    • PDCD 8 antibody
    • PDCD8 antibody
    • Programmed cell death 8 (apoptosis inducing factor) antibody
    • Programmed cell death 8 antibody
    • Programmed cell death 8 isoform 1 antibody
    • Programmed cell death 8 isoform 2 antibody
    • Programmed cell death 8 isoform 3 antibody
    • Programmed cell death protein 8 antibody
    • Programmed cell death protein 8 mitochondrial antibody
    • Programmed cell death protein 8 mitochondrial precursor antibody
    • Programmed cell death protein 8 mitochondrial precursor antibody
    • Striatal apoptosis inducing factor antibody
    see all

Images

  • All lanes : Anti-AIF antibody - C-terminal (ab205370) at 0.03 µg/ml

    Lane 1 : Mouse kidney tissue lysate in RIPA buffer
    Lane 2 : Rat kidney tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 67 kDa



    Incubated with the primary antibody for 1 hour.

  • All lanes : Anti-AIF antibody - C-terminal (ab205370) at 0.01 µg/ml

    Lane 1 : Mouse heart tissue lysate in RIPA buffer
    Lane 2 : Rat heart tissue lysate in RIPA buffer
    Lane 3 : Pig heart tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 67 kDa



    Incubated with the primary antibody for 1 hour.

  • Anti-AIF antibody - C-terminal (ab205370) at 0.1 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa



    Incubated with the primary antibody for 1 hour.

  • Anti-AIF antibody - C-terminal (ab205370) at 0.01 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

References

ab205370 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Kidney Epithelial Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
Kidney Epithelial Cells
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C

Jacob Torres

Verified customer

Submitted Apr 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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