Anti-AIFM3 antibody (ab106359)
Key features and details
- Rabbit polyclonal to AIFM3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AIFM3 antibody
See all AIFM3 primary antibodies -
Description
Rabbit polyclonal to AIFM3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AIFM3 (N terminal). 16 amino acid peptide
Database link: Q96NN9 -
Positive control
- Human tissue lysates and Human brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
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Function
Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential. -
Tissue specificity
Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level). -
Sequence similarities
Belongs to the FAD-dependent oxidoreductase family.
Contains 1 Rieske domain. -
Domain
The Rieske domain induces apoptosis. -
Cellular localization
Mitochondrion. Does not translocate to the nucleus upon induction of apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 150209 Human
- SwissProt: Q96NN9 Human
- Unigene: 723246 Human
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Alternative names
- AIFL antibody
- Aifm3 antibody
- AIFM3_HUMAN antibody
see all
Images
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Lane 1 : Anti-AIFM3 antibody (ab106359) at 1 µg/ml
Lane 2 : Anti-AIFM3 antibody (ab106359) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 67 kDa -
Immunohistochemical staining of human brain tissue using ab106359 at 2.5 µg
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Immunohistochemistry of AIFM3 in Human brain tissue with ab106359 at 20 ug/mL.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab106359 has been referenced in 1 publication.
- Araujo AN et al. Comprehensive Assessment of Copy Number Alterations Uncovers Recurrent AIFM3 and DLK1 Copy Gain in Medullary Thyroid Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33435319