Overview

  • Product name

    Anti-AIM2 antibody [3C4G11]
    See all AIM2 primary antibodies
  • Description

    Mouse monoclonal [3C4G11] to AIM2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AIM2 aa 1-195. expressed in E. Coli.
    Sequence:

    MESKYKEILL LTGLDNITDE ELDRFKFFLS DEFNIATGKL HTANRIQVAT LMIQNAGAVS AVMKTIRIFQ KLNYMLLAKR LQEEKEKVDK QYKSVTKPKP LSQAEMSPAA SAAIRNDVAK QRAAPKVSPH VKPEQKQMVA QQESIREGFQ KRCLPVMVLK AKKPFTFETQ EGKQEMFHAT VATEKEFFFV KVFNT


    Database link: O14862

  • Positive control

    • AIM2 (aa1-195) recombinant protein, AIM2 (aa1-195)-hIgGFc transfected HEK293 whole cell lysate, A549, MCF-7 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab204995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • Tissue specificity

    Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • Sequence similarities

    Belongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Absent in melanoma 2 antibody
    • AIM 2 antibody
    • Aim2 antibody
    • AIM2_HUMAN antibody
    • Interferon-inducible protein AIM2 antibody
    • OTTHUMP00000035296 antibody
    • PYHIN4 antibody
    see all

Images

  • All lanes : Anti-AIM2 antibody [3C4G11] (ab204995) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate.
    Lane 2 : AIM2 (aa1-195)-hIgGFc transfected HEK293 whole cell lysate.

    Predicted band size: 39 kDa

  • Flow cytometric analysis of HeLa cells labelling AIM2 with ab204995 (green) at a 1/200 dilution and negative control (red).

  • Immunofluorescence analysis of MCF-7 cells labeling AIM2 with ab204995 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Immunofluorescence analysis of A549 cells labeling AIM2 with ab204995 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Anti-AIM2 antibody [3C4G11] (ab204995) at 1/500 dilution + AIM2 (aa1-195) recombinant protein

    Predicted band size: 39 kDa
    Additional bands at: 48.2 kDa. We are unsure as to the identity of these extra bands.

References

ab204995 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204995.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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