Overview

  • Product name

  • Description

    Rabbit polyclonal to AIM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 93-341 of Human AIM2 (NM_004833).

  • Positive control

    • EC-109 and HepG2 whole cell lysate (ab7900); Human fetal tonsil tissue

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-AIM2 antibody (ab93015) at 1/500 dilution

    Lane 1 : EC-109 cell lysate
    Lane 2 : HepG2 cell lysate

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of fetal spleen tissue sections labeling AIM2 with ab93015 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of Jurkat cells labeling AIM2 with ab93015 at 1/200 dilution. Cells were fixed with methanol and permeabilized with citrate buffer, pH 6.0. The cells were fixed with 4% BSA for 30 minutes at 25°C, followed by incubation with Anti-AIM2 antibody (ab93015) in blocking buffer for 16 hours at 4°C. A goat-anti-rabbit FITC secondary antibody was used at 1/100 dilution.

    See Abreview

  • Immunohistochemical staining of AIM2 in formalin fixed, paraffin embedded Human fetal tonsil showing nuclear staining with ab93015 at a dilution of 1/100.
  • ab93015 staining AIM2 in human monocytes (Apheresis) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab93015 at a 1/200 dilution for 2 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

References

This product has been referenced in:

  • Botto S  et al. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1ß Protein during Virion-Mediated Activation of the AIM2 Inflammasome. MBio 10:N/A (2019). Read more (PubMed: 30755509) »
  • Shi X  et al. Dihydroartemisinin, an antimalarial drug, induces absent in melanoma 2 inflammasome activation and autophagy in human hepatocellular carcinoma HepG2215 cells. Phytother Res 33:1413-1425 (2019). Read more (PubMed: 30873702) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 10 2014

Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Loading amount
30 µg
Specification
skin
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 05 2013

Application
Immunocytochemistry
Sample
Human Cultured Cells (Jurkat)
Specification
Jurkat
Fixative
Methanol
Permeabilization
Yes - citrate buffer, pH 6,0
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 29 2013

Answer

Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab93015 has been added to your order number###. This has Abcam order reference #####.

I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vial, or if you have any further questions, please do not hesitate to let me know.

I am sorry we do not currently have information on when we will have a new batch of this antibody in stock. When the antibody is purchased again, I can recommend to contact us for an update on available lot numbers if required.

Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further.

Read More

Answer

Thank you for your email.

I do understand and appreciate the amount of time your customer hasspent it the laboratory. Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days).



Unfortunately, it looks like this order is way out of the Abpromise range. Therefore, it is hard to tell if the nonperformance is due to the product quality, storage conditions or the protocol.

I am sorry, but I am afraid I cannot help your customer in this case with a replacement or credit note. However, I would be happy to troubleshoot if they want me to.

Read More
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 19 2012

Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 01 2012

Answer

Thank you for contacting us.

I am sorry for the delay in gathering all the information that you have requested and getting back to you with it. I have contacted the suppliers of the products that you have enquired about and asked if the peptides were available for those products. The only product with an available peptide is ab115616 Anti-NALP2. I have instructed our products team to add this to our catalog and it should be up within a few days.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 16 2012

Question
Answer

Thank you for contacting us earlier, it was a pleasure speaking with you. I am sorry to hear that ab93015 is still giving you trouble.  I have set up a free of charge order for ab119845 for you, the order number is XXXXXX.  As I mentioned on the phone, this item is not currently in stock, but we will send this to you on or around December 9th. Please do let us know if you experience any further problems with this antibody.  Best of luck in your research.

Read More

1-10 of 12 Abreviews or Q&A

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