Overview

  • Product name

    Anti-AIM2 antibody - C-terminal
    See all AIM2 primary antibodies
  • Description

    Goat polyclonal to AIM2 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AIM2 aa 321-335 (C terminal) (Cysteine residue). (NP_004824.1).
    Sequence:

    C-GEKLQLTSGVHSTIK


    Database link: O14862

  • Positive control

    • WB: WI-38 cell lysate treated with IFN beta. IHC-P: Human spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223622 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50, 55 kDa (predicted molecular weight: 39 kDa).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • Tissue specificity

    Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • Sequence similarities

    Belongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Absent in melanoma 2 antibody
    • AIM 2 antibody
    • Aim2 antibody
    • AIM2_HUMAN antibody
    • Interferon-inducible protein AIM2 antibody
    • OTTHUMP00000035296 antibody
    • PYHIN4 antibody
    see all

Images

  • Paraffin-embedded human spleen tissue stained for AIM2 with ab223622 at 3.8 µg/ml in immunohistochemical analysis, followed by AP staining.

  • Anti-AIM2 antibody - C-terminal (ab223622) at 1 µg/ml + WI-38 (human fetal lung fibroblast cell line) cell lysate treated with IFN beta (RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 50,55 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223622 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223622.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up