Overview

  • Product name

    Anti-AIM2 antibody [EPR18793] (Phycoerythrin)
    See all AIM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18793] to AIM2 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ICC/IF is recommended for human only.

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human AIM2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O14862

  • Positive control

    • Flow Cyt: Daudi cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1250.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • Tissue specificity

    Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • Sequence similarities

    Belongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Absent in melanoma 2 antibody
    • AIM 2 antibody
    • Aim2 antibody
    • AIM2_HUMAN antibody
    • Interferon-inducible protein AIM2 antibody
    • OTTHUMP00000035296 antibody
    • PYHIN4 antibody
    see all

Images

  • Overlay histogram showing Daudi cells stained with ab237214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237214) (1x 106 cells in 100 µl at 0.4 µg/ml (1/1250 dilution)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

References

ab237214 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237214.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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